Chronic heart failure-induced skeletal muscle atrophy, necrosis, and changes in myogenic regulatiory factors

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dc.contributor.authorMartinez, Paula F. [UNESP]
dc.contributor.authorOkoshi, Katashi [UNESP]
dc.contributor.authorZornoff, Leonardo Antonio Mamede [UNESP]
dc.contributor.authorCarvalho, Robson F. [UNESP]
dc.contributor.authorOliveira Junior, Silvio A. [UNESP]
dc.contributor.authorLima, Aline R. R. [UNESP]
dc.contributor.authorCampos, Dijon H. S. [UNESP]
dc.contributor.authorDamatto, Ricardo L. [UNESP]
dc.contributor.authorPadovani, Carlos Roberto [UNESP]
dc.contributor.authorNogueira, Célia Regina [UNESP]
dc.contributor.authorPai-Silva, Maeli Dai [UNESP]
dc.contributor.authorOkoshi, Marina Politi [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Federal de Mato Grosso do Sul (UFMS)
dc.date.accessioned2014-05-20T13:47:57Z
dc.date.available2014-05-20T13:47:57Z
dc.date.issued2010-12-01
dc.description.abstractBackground: Although intrinsic skeletal muscle abnormalities can influence exercise intolerance during heart failure (HF), the factors responsible for muscle changes have not been elucidated. In this study we evaluated the expression of myogenic regulatory factors (MRF), myosin heavy chain (MyHC) isoforms, and fiber trophism in the soleus muscle of rats with myocardial infarction-induced heart failure.Method/Results: Six months after surgery, 2 groups of rats were studied: sham, and infarcted rats with HF (MI/HF+, MI size: 41.1 +/- 6.3% of total left ventricular area). In the infarcted group, microscopic evaluation revealed scattered foci of fiber necrosis in combination with inflammatory cells, phagocytosis, and increased fibrous tissue. The frequency of necrotic fibers was significantly higher in the MI/HF+ group than in the sham. The MI/HF+ group had atrophy of type I, IC/IIC, and HA fibers compared to the sham group (P<0.05). MyoD gene expression was higher in the MI/HF+ group (sham: 1.00 +/- 0.49; MI/HF+: 2.53 +/- 0.71 arbitrary units; P<0.001). Myogenin and MRF4 gene expression was similar in both groups. Myogenin protein levels were reduced in the MI/HF+ group (sham: 1.00 +/- 0.21; MI/HF+: 0.74 +/- 0.21 arbitrary units; P=0.026). MyoD and MRF4 protein levels, as well as the MyHC distribution, were not different between groups. The MI/HF+ group had higher TNF-alpha and IL-6 serum concentrations than the sham group.Conclusions: Heart failure-induced skeletal muscle atrophy is combined with fiber necrosis, increased MyoD gene expression and decreased myogenin protein levels.en
dc.description.affiliationUniv Estadual Paulista, Dept Internal Med, Botucatu Med Sch, São Paulo, Brazil
dc.description.affiliationUniv Estadual Paulista, Dept Morphol, Botucatu Biosci Inst, São Paulo, Brazil
dc.description.affiliationUniversidade Federal de Mato Grosso do Sul (UFMS), Sch Phys Therapy, Campo Grande, MS, Brazil
dc.description.affiliationUniv Estadual Paulista, Dept Biostat, Botucatu Biosci Inst, BR-18618000 São Paulo, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Dept Internal Med, Botucatu Med Sch, São Paulo, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Dept Morphol, Botucatu Biosci Inst, São Paulo, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Dept Biostat, Botucatu Biosci Inst, BR-18618000 São Paulo, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipFundação para o Desenvolvimento da UNESP (FUNDUNESP)
dc.description.sponsorshipIdFAPESP: 05/58294-3
dc.description.sponsorshipIdFAPESP: 05/60767-7
dc.description.sponsorshipIdCNPq: 310547/2006-7
dc.description.sponsorshipIdFUNDUNESP: 00108/06-DFP
dc.format.extentBR374-BR383
dc.identifierhttp://www.medscimonit.com/abstract/index/idArt/881289
dc.identifier.citationMedical Science Monitor. Albertson: Int Scientific Literature, Inc, v. 16, n. 12, p. BR374-BR383, 2010.
dc.identifier.issn1234-1010
dc.identifier.lattes8727897080522289
dc.identifier.lattes4463138671998432
dc.identifier.lattes1590971576309420
dc.identifier.lattes5016839015394547
dc.identifier.orcid0000-0002-4901-7714
dc.identifier.orcid0000-0001-8980-8839
dc.identifier.scopus2-s2.0-79951562366
dc.identifier.urihttp://hdl.handle.net/11449/17086
dc.identifier.wosWOS:000286361700004
dc.language.isoeng
dc.publisherInt Scientific Literature, Inc
dc.relation.ispartofMedical Science Monitor
dc.relation.ispartofsjr0,619
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.subjectmyocardial infarctionen
dc.subjectmyogenic regulatory factorsen
dc.subjectmyosin heavy chainen
dc.subjectcytokinesen
dc.titleChronic heart failure-induced skeletal muscle atrophy, necrosis, and changes in myogenic regulatiory factorsen
dc.typeArtigo
dcterms.rightsHolderInt Scientific Literature, Inc
unesp.author.lattes8727897080522289[9]
unesp.author.lattes4463138671998432
unesp.author.lattes1590971576309420
unesp.author.lattes5016839015394547[3]
unesp.author.lattes7607038776901890[10]
unesp.author.orcid0000-0002-4901-7714[4]
unesp.author.orcid0000-0001-8980-8839
unesp.author.orcid0000-0002-7719-9682[9]
unesp.author.orcid0000-0002-4014-0660[10]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatupt
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina, Botucatupt

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