Prenatal and pubertal exposure to 17α-ethinylestradiol disrupts folliculogenesis and promotes morphophysiological changes in ovaries of old gerbils (Meriones unguiculatus)

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dc.contributor.authorDe Souza, Vinícius Gonçalves
dc.contributor.authorBandeira, Laura Borges
dc.contributor.authorSouza, Nátaly Caroline Silva E
dc.contributor.authorTaboga, Sebastião Roberto [UNESP]
dc.contributor.authorMartins, Tracy Martina Marques
dc.contributor.authorSilva Perez, Ana Paula
dc.contributor.institutionUFJ
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.date.accessioned2021-06-25T10:54:22Z
dc.date.available2021-06-25T10:54:22Z
dc.date.issued2020-01-01
dc.description.abstract17α-Ethinylestradiol is an endocrine-disrupting chemical that make up most contraceptive pills and can be found in the environment. Exposure to ethinylestradiol in different development periods may promote changes in morphophysiological parameters of reproductive and endocrine organs. Considering that the effects of low doses (15 μg/kg/day) of ethinylestradiol in ovaries from 12-month-old female gerbils (Meriones unguiculatus) were investigated. Four experimental groups used were control (without treatment), EE/PRE (treated from the 18th to the 22nd gestational day), EE/PUB (treated from the 42nd to the 49th day of life), and EE/PRE-PUB (treated in the both periods). The animals were euthanized at 12 months. Testosterone and 17β-estradiol levels were measured. The ovaries were stained with Hematoxylin and Eosin, Periodic Acid Schiff, and Gomori's Trichome. The follicles, corpus luteum, interstitial gland, lipofuscin, ovarian epithelium, and tunica albuginea were analyzed. Estradiol was higher in EE/PRE and EE/PUB groups, while testosterone was higher only in EE/PUB group. The main changes in follicle count occurred in EE/PUB and EE/PRE-PUB groups, with higher primordial follicle count and lower maturation of follicles. The corpus luteum was more evident in EE/PRE group. No differences were found in atretic follicles count. A higher area occupied by interstitial gland cells and lipofuscin deposit in these cells was noted in EE/PUB and EE/PRE-PUB groups. Higher epithelium height and thicker tunic albuginea were showed in treated groups. These results suggest that exposure to doses of EE2 in prenatal and pubertal periods of the development leads to morphological changes in senile ovaries.en
dc.description.affiliationInstitute of Health Sciences Medicine Course Federal University of Jataí UFJ
dc.description.affiliationDepartment of Biology Laboratory of Microscopy and Microanalysis São Paulo State University-UNESP
dc.description.affiliationDepartment of Structural and Functional Biology State University of Campinas UNICAMP
dc.description.affiliationInstitute of Health Sciences Medicine Course and Graduate Program of Animal Bioscience Federal University of Jataí UFJ
dc.description.affiliationUnespDepartment of Biology Laboratory of Microscopy and Microanalysis São Paulo State University-UNESP
dc.identifierhttp://dx.doi.org/10.1017/S2040174421000040
dc.identifier.citationJournal of Developmental Origins of Health and Disease.
dc.identifier.doi10.1017/S2040174421000040
dc.identifier.issn2040-1752
dc.identifier.issn2040-1744
dc.identifier.scopus2-s2.0-85101960684
dc.identifier.urihttp://hdl.handle.net/11449/207387
dc.language.isoeng
dc.relation.ispartofJournal of Developmental Origins of Health and Disease
dc.sourceScopus
dc.subjectcontraceptive agents
dc.subjectendocrine disruptors
dc.subjectGerbillinae
dc.subjectKeywords:
dc.subjectovary
dc.titlePrenatal and pubertal exposure to 17α-ethinylestradiol disrupts folliculogenesis and promotes morphophysiological changes in ovaries of old gerbils (Meriones unguiculatus)en
dc.typeArtigo

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