Pseudechis guttatus venom proteome: Insights into evolution and toxin clustering

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dc.contributor.authorViala, Vincent L.
dc.contributor.authorHildebrand, Diana
dc.contributor.authorTrusch, Maria
dc.contributor.authorArni, Raghuvir K. [UNESP]
dc.contributor.authorPimenta, Daniel C.
dc.contributor.authorSchlueter, Hartmut
dc.contributor.authorBetzel, Christian
dc.contributor.authorSpencer, Patrick J.
dc.contributor.institutionIPEN
dc.contributor.institutionUniv Klinikum Hamburg Eppendorf
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionInst Butantan
dc.contributor.institutionUniv Hamburg
dc.date.accessioned2015-03-18T15:53:38Z
dc.date.available2015-03-18T15:53:38Z
dc.date.issued2014-10-14
dc.description.abstractThe Australian Elapidae spotted black snake Pseudechis guttatus venom proteome composition was analyzed by high throughput mass spectrometry. The crude venom proteins were decomplexed by 2D-PAGE and in-gel digestion peptides from 66 spot samples and analyzed by tandem mass spectrometry-LC-ESI-ion trap. Protein identification was performed combining PEAKS studio 7.0 and Mascot software. The analysis identified L-amino-acid oxidases, phospholipases A2, metalloproteases, nerve growth factors and ecto-5'-nucleotidases, and for the first time in this venom the components cysteine-rich secretory proteins similar to pseudechetoxin, phospholipase B and transferrin-like protein. The envenomation symptoms are in agreement with the identified components, but the present limitations of database information might impair the detection of toxin families, protein species and still unknown toxins. From the qualitative point of view, the similarity of this venom with the ones from other Pseudechis species could be assigned to recent speciation events.Biological significanceStudies on the proteome of Australian Elapidae (Ancanthophiinae) are quite rare. In the present work we performed, using classic proteomic methods, a qualitative and partial analysis of the proteic components of Pseudechis guttatus venom. Although previous studies contributed to the knowledge of the major components of this venom, our study revealed some yet undescribed protein species, as well as new toxins, such as CRiSPs, phospholipase B, transferrin-like protein and ecto 5'-nucleotidase. (C) 2014 Elsevier B.V. All rights reserved.en
dc.description.affiliationIPEN, Ctr Biotechnol, BR-05508000 Sao Paulo, Brazil
dc.description.affiliationUniv Klinikum Hamburg Eppendorf, Inst Klin Chem, D-20246 Hamburg, Germany
dc.description.affiliationUNESP, IBILCE, Dept Fis, BR-15054000 Sao Jose Do Rio Preto, SP, Brazil
dc.description.affiliationInst Butantan, Lab Bioquim & Biofis, BR-05503900 Sao Paulo, Brazil
dc.description.affiliationUniv Hamburg, Inst Biochem & Mol Biol, D-20146 Hamburg, Germany
dc.description.affiliationUnespUNESP, IBILCE, Dept Fis, BR-15054000 Sao Jose Do Rio Preto, SP, Brazil
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdCAPES: 364/11
dc.description.sponsorshipIdFAPESP: 09/1305-8
dc.format.extent32-44
dc.identifierhttp://dx.doi.org/10.1016/j.jprot.2014.07.030
dc.identifier.citationJournal Of Proteomics. Amsterdam: Elsevier Science Bv, v. 110, p. 32-44, 2014.
dc.identifier.doi10.1016/j.jprot.2014.07.030
dc.identifier.issn1874-3919
dc.identifier.lattes9162508978945887
dc.identifier.orcid0000-0003-2460-1145
dc.identifier.urihttp://hdl.handle.net/11449/116634
dc.identifier.wosWOS:000345183200004
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofJournal Of Proteomics
dc.relation.ispartofjcr3.722
dc.relation.ispartofsjr1,430
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectPseudechis guttatusen
dc.subjectElapidaeen
dc.subjectToxinsen
dc.subjectHigh throughputen
dc.subjectProteomicsen
dc.subject2D-PAGEen
dc.titlePseudechis guttatus venom proteome: Insights into evolution and toxin clusteringen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.
unesp.author.lattes9162508978945887[4]
unesp.author.orcid0000-0003-2406-0860[5]
unesp.author.orcid0000-0002-9358-7036[6]
unesp.author.orcid0000-0003-2460-1145[4]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências Letras e Ciências Exatas, São José do Rio Pretopt

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