Effect of combined fish oil & Curcumin on murine skeletal muscle morphology and stress response proteins during mechanical unloading

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dc.contributor.authorLawler, John M.
dc.contributor.authorGarcia-Villatoro, Erika L.
dc.contributor.authorGuzzoni, Vinicius
dc.contributor.authorHord, Jeff M.
dc.contributor.authorBotchlett, Rachel
dc.contributor.authorHolly, Dylan
dc.contributor.authorLawler, Matthew S.
dc.contributor.authorJanini Gomes, Mariana [UNESP]
dc.contributor.authorRyan, Pat
dc.contributor.authorRodriguez, Dinah
dc.contributor.authorKuczmarski, J. Matthew
dc.contributor.authorFluckey, James D.
dc.contributor.authorTalcott, Susanne
dc.contributor.institutionTexas A&M University
dc.contributor.institutionFederal University of Paraíba
dc.contributor.institutionUniversity of Iowa
dc.contributor.institutionGeorgia Tech University
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2019-10-06T16:23:24Z
dc.date.available2019-10-06T16:23:24Z
dc.date.issued2019-05-01
dc.description.abstractSkeletal muscle is a highly adaptable tissue capable of remodeling when dynamic stress is altered, including changes in mechanical loading and stretch. When muscle is subjected to an unloaded state (e.g., bedrest, immobilization, spaceflight) the resulting loss of muscle cross sectional area (CSA) impairs force production. In addition, muscle fiber-type shifts from slow to fast-twitch fibers. Unloading also results in a downregulation of heat shock proteins (e.g., HSP70) and anabolic signaling, which further exacerbate these morphological changes. Our lab recently showed reactive oxygen species (ROS) are causal in unloading-induced alterations in Akt and FoxO3a phosphorylation, muscle fiber atrophy, and fiber-type shift. Nutritional supplements such as fish oil and curcumin enhance anabolic signaling, glutathione levels, and heat shock proteins. We hypothesized that fish oil, rich in omega-3-fatty acids, combined with the polyphenol curcumin would enhance stress protective proteins and anabolic signaling in the rat soleus muscle, concomitant with synergistic protection of morphology. C57BL/6 mice were assigned to 3 groups (n = 6/group): ambulatory controls (CON), hindlimb unloading (HU), and hindlimb unloading with 5% fish oil, 1% curcumin in diet (FOC). FOC treatments began 10 days prior to HU and tissues were harvested following 7 days of HU. FOC mitigated the unloading induced decrease in CSA. FOC also enhanced abundance of HSP70 and anabolic signaling (Akt phosphorylation, p70S6K phosphorylation), while reducing Nox2, a source of oxidative stress. Therefore, we concluded that the combination of fish oil and curcumin prevents skeletal muscle atrophy due to a boost of heat shock proteins and anabolic signaling in an unloaded state.en
dc.description.affiliationRedox Biology & Cell Signaling Laboratory Department of Health and Kinesiology Texas A&M University
dc.description.affiliationDepartment of Nutrition and Food Science Texas A&M University
dc.description.affiliationDepartment of Cellular and Molecular Biology Federal University of Paraíba
dc.description.affiliationDepartment of Molecular Physiology and Biophysics Carver School of Medicine University of Iowa
dc.description.affiliationDepartment of Biomedical Engineering Georgia Tech University
dc.description.affiliationDepartment of Internal Medicine Botucatu Medical School São Paulo State University (UNESP)
dc.description.affiliationMuscle Biology Laboratory Department of Health and Kinesiology Texas A&M University
dc.description.affiliationUnespDepartment of Internal Medicine Botucatu Medical School São Paulo State University (UNESP)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipNational Center for Research Resources
dc.description.sponsorshipNorges Idrettshøgskole
dc.description.sponsorshipNational Aeronautics and Space Administration
dc.description.sponsorshipNational Space Biomedical Research Institute
dc.description.sponsorshipIdNational Center for Research Resources: 1 S10 RR22532-01
dc.description.sponsorshipIdNorges Idrettshøgskole: AR054084
dc.description.sponsorshipIdNational Aeronautics and Space Administration: NNX13AE45G
dc.format.extent17-28
dc.identifierhttp://dx.doi.org/10.1016/j.nutres.2018.12.013
dc.identifier.citationNutrition Research, v. 65, p. 17-28.
dc.identifier.doi10.1016/j.nutres.2018.12.013
dc.identifier.issn1879-0739
dc.identifier.issn0271-5317
dc.identifier.scopus2-s2.0-85063724504
dc.identifier.urihttp://hdl.handle.net/11449/188921
dc.language.isoeng
dc.relation.ispartofNutrition Research
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectAnabolic
dc.subjectAtrophy
dc.subjectHSP70
dc.subjectNox2
dc.subjectNutraceutical
dc.subjectSkeletal Muscle
dc.subjectSpaceflight
dc.titleEffect of combined fish oil & Curcumin on murine skeletal muscle morphology and stress response proteins during mechanical unloadingen
dc.typeArtigo
unesp.author.orcid0000-0003-1024-5952 0000-0003-1024-5952[3]

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