Atividade tóxica de isolados de Bacillus Thuringiensis a larvas de Aedes Aegypti (l.) (diptera: culicidae)

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dc.contributor.authorCosta, Juliana R V da
dc.contributor.authorRossi, Juliana R
dc.contributor.authorMarucci, Suzana C
dc.contributor.authorAlves, Eliane C da C
dc.contributor.authorVolpe, Haroldo X L
dc.contributor.authorFerraudo, Antonio S [UNESP]
dc.contributor.authorLemos, Manoel V F
dc.contributor.authorDesidério, Janete A
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T13:13:49Z
dc.date.available2014-05-20T13:13:49Z
dc.date.issued2010-10-01
dc.description.abstractAedes aegypti (L.), the main vector of dengue fever in Brazil, has been controlled with the use of massive chemical products, contributing to the development of resistance and decreasing the insect control efficiency. The control of dipterans with bioinsecticides based on Bacillus thuringiensis has been satisfactory, due to the production of insecticidal proteins denominated Cry (crystal), Cyt (cytolytic) toxins and Chi (chitinase), and to the synergistic effects among them. The present work aimed to select B. thuringiensis isolates efficient against A. aegypti larvae. A bacterial collection containing 1,073 isolates of B. thuringiensis, obtained from different locations of Brazilian territory, had the DNA isolated and submitted to PCR amplifications using specific primers for cry4Aa, cry4Ba, cry11Aa, cry11Ba, cyt1Aa, cyt1Ab, cyt2Aa and chi genes. For the LC50 and LC90 determination, the entomopathogenic isolates were evaluated by selective and quantitative bioassays. Only 45 isolates (4.2%) presented amplicons for the cry and cyt genes. The chi gene sequence was detected in 25 (54.3%) of those isolates. From the 45 isolates submitted to the selective bioassays, 13 caused 100% mortality of A. aegypti larvae. The identification of cry, cyt and chi genes of B. thuringiensis and the toxicity analysis on A. aegypti led to the selection of a set of isolates that have the potential to be used in the formulation of new bioinsecticides.en
dc.description.affiliationUnesp Faculdade de Ciências Agrárias e Veterinárias Depto de Ciências Exatas
dc.description.affiliationUnespUnesp Faculdade de Ciências Agrárias e Veterinárias Depto de Ciências Exatas
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.format.extent757-766
dc.identifierhttp://dx.doi.org/10.1590/S1519-566X2010000500015
dc.identifier.citationNeotropical Entomology. Sociedade Entomológica do Brasil, v. 39, n. 5, p. 757-766, 2010.
dc.identifier.doi10.1590/S1519-566X2010000500015
dc.identifier.fileS1519-566X2010000500015.pdf
dc.identifier.issn1519-566X
dc.identifier.lattes1295051340591836
dc.identifier.scieloS1519-566X2010000500015
dc.identifier.urihttp://hdl.handle.net/11449/1487
dc.identifier.wosWOS:000284502300015
dc.language.isoeng
dc.publisherSociedade Entomológica do Brasil
dc.relation.ispartofNeotropical Entomology
dc.relation.ispartofjcr0.886
dc.rights.accessRightsAcesso aberto
dc.sourceSciELO
dc.subjectBiological controlen
dc.subjectCryen
dc.subjectCyten
dc.subjectChitinaseen
dc.subjectDengue feveren
dc.titleAtividade tóxica de isolados de Bacillus Thuringiensis a larvas de Aedes Aegypti (l.) (diptera: culicidae)en
dc.title.alternativeToxic activity of Bacillus Thuringiensis isolates to Aedes Aegypti (l.) (diptera: culicidae) larvaeen
dc.typeArtigo
dcterms.licensehttp://www.scielo.br/revistas/ne/paboutj.htm
unesp.author.lattes1295051340591836
unesp.author.lattes7159757610060958[6]
unesp.author.orcid0000-0001-7723-3070[8]
unesp.author.orcid0000-0003-3092-4741[5]
unesp.author.orcid0000-0002-7089-923X[6]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Agrárias e Veterinárias, Jaboticabalpt

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