Biostimulatory effect of low-level laser therapy on keratinocytes in vitro

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dc.contributor.authorBasso, Fernanda G.
dc.contributor.authorOliveira, Camila F. [UNESP]
dc.contributor.authorKurachi, Cristina
dc.contributor.authorHebling, Josimeri [UNESP]
dc.contributor.authorCosta, Carlos Alberto de Souza [UNESP]
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2014-05-27T11:28:17Z
dc.date.available2014-05-27T11:28:17Z
dc.date.issued2013-02-01
dc.description.abstractEpithelial cells play an important role in reparative events. Therefore, therapies that can stimulate the proliferation and metabolism of these cells could accelerate the healing process. To evaluate the effects of low-level laser therapy (LLLT), human keratinocytes were irradiated with an InGaAsP diode laser prototype (LASERTable; 780 ± 3 nm; 40 mW) using 0.5, 1.5, 3, 5, and 7 J/cm2 energy doses. Irradiations were done every 24 h totaling three applications. Evaluation of cell metabolism (MTT assay) showed that LLLT with all energy doses promoted an increase of cell metabolism, being more effective for 0.5, 1.5, and 3 J/cm2. The highest cell counts (Trypan blue assay) were observed with 0.5, 3, and 5 J/cm2. No statistically significant difference for total protein (TP) production was observed and cell morphology analysis by scanning electron microscopy revealed that LLLT did not promote morphological alterations on the keratinocytes. Real-time polymerase chain reaction (qPCR) revealed that LLLT also promoted an increase of type I collagen (Col-I) and vascular endothelial growth factor (VEGF) gene expression, especially for 1.5 J/cm2, but no change on fibroblast growth factor-2 (FGF-2) expression was observed. LLLT at energy doses ranging from 0.5 to 3 J/cm2 promoted the most significant biostimulatory effects on cultured keratinocytes. © 2012 Springer-Verlag London Ltd.en
dc.description.affiliationPiracicaba School of Dentistry UNICAMP - University of Campinas, Piracicaba, SP 13414-903
dc.description.affiliationAraraquara School of Dentistry UNESP - Univ. Estadual Paulista, Araraquara, SP 14801-903
dc.description.affiliationPhysics Institute of São Carlos USP - University of São Paulo, São Carlos SP 13560-970
dc.description.affiliationUnespAraraquara School of Dentistry UNESP - Univ. Estadual Paulista, Araraquara, SP 14801-903
dc.format.extent367-374
dc.identifierhttp://dx.doi.org/10.1007/s10103-012-1057-8
dc.identifier.citationLasers in Medical Science, v. 28, n. 2, p. 367-374, 2013.
dc.identifier.doi10.1007/s10103-012-1057-8
dc.identifier.issn0268-8921
dc.identifier.issn1435-604X
dc.identifier.lattes4517484241515548
dc.identifier.scopus2-s2.0-84878929165
dc.identifier.urihttp://hdl.handle.net/11449/74449
dc.identifier.wosWOS:000315579400003
dc.language.isoeng
dc.relation.ispartofLasers in Medical Science
dc.relation.ispartofjcr1.949
dc.relation.ispartofsjr0,713
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subjectEpithelial cells
dc.subjectGene expression
dc.subjectLow-level laser therapy
dc.subjectcell protein
dc.subjectcollagen type 1
dc.subjectfibroblast growth factor 2
dc.subjectvasculotropin
dc.subjectcell count
dc.subjectcell metabolism
dc.subjectcell stimulation
dc.subjectcell structure
dc.subjectcell transformation
dc.subjectcontrolled study
dc.subjectdiode laser
dc.subjectgene expression
dc.subjecthuman
dc.subjecthuman cell
dc.subjectin vitro study
dc.subjectirradiation
dc.subjectkeratinocyte
dc.subjectlow level laser therapy
dc.subjectmetabolic rate
dc.subjectpriority journal
dc.subjectprotein expression
dc.subjectprotein synthesis
dc.subjectradiation dose
dc.subjecttype I collagen gene
dc.subjectvascular endothelial growth factor gene
dc.titleBiostimulatory effect of low-level laser therapy on keratinocytes in vitroen
dc.typeArtigo
dcterms.licensehttp://www.springer.com/open+access/authors+rights
unesp.author.lattes4517484241515548[5]
unesp.author.orcid0000-0002-7455-6867[5]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Odontologia, Araraquarapt

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