Diagnosis of uterine and vaginal disorders by different methodologies is affected by concentration of estradiol in plasma

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dc.contributor.authorSilper, B. F.
dc.contributor.authorMadureira, A. M. L.
dc.contributor.authorBurnett, T. A.
dc.contributor.authorFernandes, A. C. C.
dc.contributor.authorAbreu, F. M.
dc.contributor.authorVeira, D. M.
dc.contributor.authorVasconcelos, J. L. M. [UNESP]
dc.contributor.authorCerri, R. L. A.
dc.contributor.institutionUniv British Columbia
dc.contributor.institutionOhio State Univ
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2018-11-26T16:32:59Z
dc.date.available2018-11-26T16:32:59Z
dc.date.issued2016-06-01
dc.description.abstractThe relationship between plasma estradiol concentration at time of examination and prevalence of uterine disorders, agreement among methods, and associations of diagnosis with pregnancy hazard and milk yield was studied in 268 Holstein cows examined at 30 +/- 3 (exam 1) and 44 +/- 3 d in milk (DIM; exam 2). Purulent vaginal discharge was sampled using 2 methods: gloved hand and Metricheck (Simcro, Hamilton, New Zealand; PVD; score >= 3). Percentage of polymorphonuclear leukocytes was determined by endometrial cytology (CYTO; exam 1: >= 18%, exam 2: >= 10%); diameter of uterine horns (UTH; >20 mm), diameter of the inner layer of the cervix (CVX; >20.5 mm), presence of fluid in the uterine lumen (FL), and ovarian structures were evaluated by ultrasonography. A blood sample was collected at each exam for estradiol analysis. Prevalence at exams 1 and 2 was, respectively, 14.2 and 18.5% (PVD), 21.4 and 10.1% (FL), and 40.6 and 50.2% (CYTO). Prevalence of PVD at exam 1 was greater among cows with estradiol >= 2 pg/mL (19.4 vs. 8.2%). Agreement of all methods with CYTO was poor, the greatest being between CYTO and FL (exam 1; kappa = 0.19). Agreement between CYTO and PVD, and between CYTO and FL (exam 1; kappa = 0.15 and 0.35, respectively) was higher among cows with estradiol >= 2 pg/mL. Likelihood of PVD at exam 1 was greater if cows were positive for CVX [odds ratio (OR) = 3.0], FL (OR = 2.6) or had estradiol >= 2 pg/mL (OR = 2.7). Likelihood of CYTO increased with dystocia (OR = 2.3) and FL (OR = 2.5). Estradiol did not influence diagnosis at exam 2. Positive FL or CYTO at exam 1 was associated with reductions in milk yield of 59 to 180 kg by 45 DIM. Pregnancy hazard until 250 DIM was reduced by CYTO at exam 1 (hazard ratio = 0.74) and by PVD (hazard ratio = 0.68) at exam 2. However, FL and CYTO reduced pregnancy hazard only when estradiol was >= 2 pg/mL (exam 1), whereas PVD reduced pregnancy hazard when diagnosed at exam 2 with estradiol <2 pg/mL. Overall, agreement was poor and effects of positive diagnosis differed according to method and DIM at exam. Estradiol concentration influenced prevalence, agreement, likelihood of positive diagnosis, and its effects on days to pregnancy.en
dc.description.affiliationUniv British Columbia, Fac Land & Food Syst, Vancouver, BC V6T 1Z4, Canada
dc.description.affiliationOhio State Univ, Dept Anim Sci, Columbus, OH 43210 USA
dc.description.affiliationSao Paulo State Univ, Dept Anim Prod, BR-18168000 Botucatu, SP, Brazil
dc.description.affiliationUnespSao Paulo State Univ, Dept Anim Prod, BR-18168000 Botucatu, SP, Brazil
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipDairy Research Cluster Initiative (Dairy Farmers of Canada)
dc.description.sponsorshipDairy Research Cluster Initiative (Agriculture and Agri-Food Canada)
dc.description.sponsorshipDairy Research Cluster Initiative (Canadian Dairy Network)
dc.description.sponsorshipDairy Research Cluster Initiative (Canadian Dairy Commission; Ottawa, ON, Canada)
dc.description.sponsorshipNatural Sciences and Engineering Research Council of Canada (Ottawa, ON, Canada)
dc.format.extent4795-4807
dc.identifierhttp://dx.doi.org/10.3168/jds.2015-10483
dc.identifier.citationJournal Of Dairy Science. New York: Elsevier Science Inc, v. 99, n. 6, p. 4795-4807, 2016.
dc.identifier.doi10.3168/jds.2015-10483
dc.identifier.issn0022-0302
dc.identifier.urihttp://hdl.handle.net/11449/161498
dc.identifier.wosWOS:000375876600065
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofJournal Of Dairy Science
dc.relation.ispartofsjr1,350
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectcytology
dc.subjectendometritis
dc.subjectestradiol
dc.subjectfertility
dc.titleDiagnosis of uterine and vaginal disorders by different methodologies is affected by concentration of estradiol in plasmaen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.

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