Proteoglycans orchestrate remodeling of prostatic cytoarchitecture after androgenic blockade in old gerbils

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dc.contributor.authorCampos, Silvana G. P. [UNESP]
dc.contributor.authorGonçalves, Bianca F. [UNESP]
dc.contributor.authorRuiz, Thalles Fernando Rocha
dc.contributor.authorLeonel, Ellen Cristina R.
dc.contributor.authorRibeiro, Daniele L.
dc.contributor.authorFalleiros Junior, Luiz Roberto
dc.contributor.authorGoes, Rejane M. [UNESP]
dc.contributor.authorTaboga, Sebastião R. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.contributor.institutionUniversidade Federal de Goiás (UFG)
dc.contributor.institutionUniversidade Federal de Uberlândia (UFU)
dc.contributor.institutionSão José Rio Preto School of Medicine (FAMERP)
dc.date.accessioned2023-07-29T13:59:24Z
dc.date.available2023-07-29T13:59:24Z
dc.date.issued2023-02-01
dc.description.abstractBackground: The aim of this study was to evaluate modifications in proteoglycan morphology and composition in the prostatic stroma of 18-month-old gerbils after surgical castration, in association or not with an androgenic blockade. Methods: The animals (n = 5) were sorted into groups subjected or not to antiandrogen treatment (flutamide 10 mg/kg/day) administered for the total postsurgery period and euthanized at 7- or 30-day postcastration; the control group consisted of intact animals. Tissue analysis included immunohistochemical assessment (perlecan and chondroitin sulfate) and proteoglycan morphology was analyzed by transmission electron microscopy. Results: Chondroitin sulfate frequency was increased 7 days postcastration with an androgenic blockade. The presence of these carbohydrates was rare after 30 days of androgenic blockade treatment. There was a significant increase in the amount of perlecan in the prostate stroma from groups subjected to castration plus flutamide for 7 or 30 days. Ultrastructural analysis showed that the incidence of areas occupied by proteoglycans and basement membrane was altered by treatment. In addition, androgenic blockade results in changes in the amount, thickness, and morphology of these structures. At 30 days postcastration, with or without flutamide treatment, larger proteoglycans were common. Conclusions: In this study, in particular, the decrease in chondroitin sulfate after the longer period might be understood as a prostatic response to androgenic deprivation, while the high frequency and permanence of perlecan led to the assumption that its modulation could be androgen-independent. Length and form alterations in proteoglycans as well as associations among them and with the basement membrane were dynamic events in the prostate microenvironment.en
dc.description.affiliationDepartment of Biological Sciences Institute of Biosciences Humanities and Exact Sciences São Paulo State University (Unesp), São Paulo
dc.description.affiliationDepartment of Structural and Functional Biology State University of Campinas (UNICAMP), São Paulo
dc.description.affiliationDepartment of Histology Embryology and Cell Biology Institute of Biological Sciences Federal University of Goiás (UFG), Goiás
dc.description.affiliationDepartment of Cell Biology Histology and Embriology Institute of Biomedical Sciences-ICBIM Federal University of Uberlândia (UFU), Minas Gerais
dc.description.affiliationDepartment of Urology São José Rio Preto School of Medicine (FAMERP), São Paulo
dc.description.affiliationUnespDepartment of Biological Sciences Institute of Biosciences Humanities and Exact Sciences São Paulo State University (Unesp), São Paulo
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.format.extent179-189
dc.identifierhttp://dx.doi.org/10.1002/pros.24451
dc.identifier.citationProstate, v. 83, n. 2, p. 179-189, 2023.
dc.identifier.doi10.1002/pros.24451
dc.identifier.issn1097-0045
dc.identifier.issn0270-4137
dc.identifier.scopus2-s2.0-85140094371
dc.identifier.urihttp://hdl.handle.net/11449/248993
dc.language.isoeng
dc.relation.ispartofProstate
dc.sourceScopus
dc.subjectcastration
dc.subjectchondroitin sulfate
dc.subjectflutamide
dc.subjectperlecan
dc.subjectprostate
dc.titleProteoglycans orchestrate remodeling of prostatic cytoarchitecture after androgenic blockade in old gerbilsen
dc.typeArtigo
unesp.author.orcid0000-0002-6813-7148[1]
unesp.author.orcid0000-0002-3622-460X[7]
unesp.author.orcid0000-0002-0970-4288[8]

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