A presença da proteína nuclear específica neuronal (NeuN) no sistema de temporização circadiano do macaco capuchinho (Cebus apella)

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dc.contributor.authordo Nascimento, Rayane Bartira Silva
dc.contributor.authorBorda, Janaína Siqueira
dc.contributor.authorEngelberth, Rovena Clara Galvão Januário
dc.contributor.authorde Medeiros, Raysa Oliveira
dc.contributor.authorFrazão, Renata
dc.contributor.authorPinato, Luciana
dc.contributor.authorde Pontes, André Luiz Bezerra
dc.contributor.authordo Nascimento Jr., Expedito Silva
dc.contributor.authorNogueira, Maria Inês
dc.contributor.authorCruz-Rizzolo, Roelf Justino [UNESP]
dc.contributor.authorCosta, Miriam Stela Maris de Oliveira
dc.contributor.authorCavalcante, Jeferson de Souza
dc.contributor.institutionUniversidade Federal do Rio Grande do Norte - UFRN
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.date.accessioned2022-04-28T22:02:07Z
dc.date.available2022-04-28T22:02:07Z
dc.date.issued2010-12-01
dc.description.abstractBackground and objective: The circadian timing system (CTS) is composed of a group of specialized neuronal structures that establish a temporal organization of physiological and behavioral processes within precise patterns. The central components of this system are the suprachiasmatic nucleus of the hypothalamus (SCN) and the intergeniculate leaflet of the thalamus (IGL). The objective of this study was to verify the presence of the neuron-specific nuclear protein (NeuN) in the circadian timing system of capuchin monkeys (Cebus apella) using immunohistochemical techniques. Methods: Capuchin monkeys (Cebus apella) were anesthetized and transcardially perfused with 4% paraformaldehyde in 0.1 M phosphate buffer, and then their brains were removed and frozen. A microtome was used to make 30 μm sections in the coronal plane. One of the series was used for Nissl staining (thionin) to demarcate the cytoarchitecture, and the remainders of the sections were processed for immunohistochemical detection of NeuN (ABC protocol). Results: NeuN-positive neurons were observed in the suprachiasmatic nucleus of the capuchin monkey. The pregeniculate nucleus (PGN), a structure equivalent to the ventral lateral geniculate nucleus (vLGN) and to the IGL in rodents, did not have any NeuN-positive neurons. Conclusions: In this primate species, only the suprachiasmatic nucleus neurons of the central structures of the circadian timing system express the NeuN protein.en
dc.description.affiliationLaboratório de Cronobiologia Centro de Biociências Universidade Federal do Rio Grande do Norte - UFRN, Natal (RN)
dc.description.affiliationDepartamento de Anatomia Universidade de São Paulo - USP, São Paulo (SP)
dc.description.affiliationDepartamento de Ciências Básicas Universidade Estadual Paulista 'Júlio de Mesquita Filho' - UNESP, Araçatuba (SP)
dc.description.affiliationUnespDepartamento de Ciências Básicas Universidade Estadual Paulista 'Júlio de Mesquita Filho' - UNESP, Araçatuba (SP)
dc.format.extent36-39
dc.identifier.citationSleep Science, v. 3, n. 1, p. 36-39, 2010.
dc.identifier.issn1984-0659
dc.identifier.issn1984-0063
dc.identifier.scopus2-s2.0-78651458232
dc.identifier.urihttp://hdl.handle.net/11449/226203
dc.language.isoeng
dc.language.isopor
dc.relation.ispartofSleep Science
dc.sourceScopus
dc.subjectAnimals
dc.subjectCebus
dc.subjectCircadian rhythm
dc.subjectGeniculate bodies
dc.subjectImmunohistochemistry/methods
dc.subjectNeurons
dc.subjectNuclear proteins
dc.subjectSuprachiasmatic nucleus
dc.titleA presença da proteína nuclear específica neuronal (NeuN) no sistema de temporização circadiano do macaco capuchinho (Cebus apella)pt
dc.title.alternativeThe presence of neuronal-specific nuclear protein (NeuN) in the circadian timing system of the capuchin monkey (Cebus apella)en
dc.typeArtigo

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