Virulence markers associated with Trueperella pyogenes infections in livestock and companion animals

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dc.contributor.authorRisseti, R. M. [UNESP]
dc.contributor.authorZastempowska, E.
dc.contributor.authorTwarużek, M.
dc.contributor.authorLassa, H.
dc.contributor.authorPantoja, J. C.F. [UNESP]
dc.contributor.authorde Vargas, A. P.C.
dc.contributor.authorGuerra, S. T. [UNESP]
dc.contributor.authorBolaños, C. A.D. [UNESP]
dc.contributor.authorde Paula, C. L. [UNESP]
dc.contributor.authorAlves, A. C. [UNESP]
dc.contributor.authorColhado, B. S. [UNESP]
dc.contributor.authorPortilho, F. V.R. [UNESP]
dc.contributor.authorTasca, C.
dc.contributor.authorLara, G. H.B. [UNESP]
dc.contributor.authorRibeiro, M. G. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionKazimierz Wielki University
dc.contributor.institutionMilk Testing Laboratory
dc.contributor.institutionUniversidade Federal de Sergipe (UFS)
dc.date.accessioned2018-12-11T17:13:14Z
dc.date.available2018-12-11T17:13:14Z
dc.date.issued2017-08-01
dc.description.abstractTrueperella pyogenes is an opportunistic pathogen that causes diverse pyogenic infections in livestock. The genes that encode the exotoxin pyolysin (plo) and other putative factors that promote adhesion of pathogen to host cells (fimbriae fimA, fimC, fimE, fimG, neuraminidases nanH, nanP, and collagen-binding protein cbpA) have been associated with virulence, particularly in mastitis and uterus infections of dairy cows. However, the role of these virulence markers in the pathogenicity of the agent in domestic animals infections still is incompletely understood. The genes plo, fimA, fimC, fimE, fimG, nanH, nanP, and cbpA were investigated in 71 T. pyogenes strains recovered from cattle, sheep, goats, dogs, equines, and a pig, recovered from mastitis (n = 35), and non-mastitis (n = 36) cases (abscesses, reproductive tract diseases, pneumonia, lymphadenitis, encephalitis). The most common genes harboured by the isolates were: plo (71/71 = 100·0%), fimA (70/71 = 98·6%), nanP (56/71 = 78·9%), fimE (53/71 = 74·6%), fimC (46/71 = 64·8%) and nanH (45/71 = 63·4%), whereas cbpA (6/71 = 8·4%) and fimG (4/71 = 5·6%) were uncommon. The most frequent genotypes were plo/fimA/fimE/fimC/nanH/nanP (17/71 = 23·9%), plo/fimA/fimE/nanH/nanP (13/71 = 18·3%), and plo/fimA/fimE/fimC/nanP (11/71 = 15·5%). No association was observed between the presence of genes vs clinical signs or host species. To the best of our knowledge, this is the first report on aforementioned virulence factors of pathogen detected in diseased horses and dogs. Significance and Impact of the Study: The role of particular virulence factors of Trueperella pyogenes that determine different pyogenic infections among domestic animals is poorly understood. Eight putative virulence genes and genotype profiles of 71 isolates were investigated among different clinical manifestations in domestic animals. The most common genes were plo (71/71 = 100·0%), fimA (70/71 = 98·6%), nanP (56/71 = 78·9%), fimE (53/71 = 74·6%), fimC (46/71 = 64·8%) and nanH (45/71 = 63·4%), whereas plo/fimA/fimE/fimC/nanH/nanP (17/71 = 23·9%), plo/fimA/fimE/nanH/nanP (13/71 = 18·3%), and plo/fimA/fimE/fimC/nanP (11/71 = 15·5%) were the most frequent genotypes. Studies involving virulence factors are critical in the investigation of molecular epidemiology, pathogenicity, and hypothetical differences in the virulence among T. pyogenes strains from different geographical areas.en
dc.description.affiliationDepartment of Veterinary Hygiene and Public Health School of Veterinary Medicine and Animal Sciences UNESP - São Paulo State University
dc.description.affiliationDepartment of Physiology and Toxicology Institute of Experimental Biology Faculty of Natural Sciences Kazimierz Wielki University
dc.description.affiliationMilk Testing Laboratory
dc.description.affiliationDepartment of Preventive Veterinary Medicine Federal University of Santa Maria-UFSM
dc.description.affiliationUnespDepartment of Veterinary Hygiene and Public Health School of Veterinary Medicine and Animal Sciences UNESP - São Paulo State University
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipIdCNPq: 304529/2013-3
dc.format.extent125-132
dc.identifierhttp://dx.doi.org/10.1111/lam.12757
dc.identifier.citationLetters in Applied Microbiology, v. 65, n. 2, p. 125-132, 2017.
dc.identifier.doi10.1111/lam.12757
dc.identifier.issn1472-765X
dc.identifier.issn0266-8254
dc.identifier.scopus2-s2.0-85022215499
dc.identifier.urihttp://hdl.handle.net/11449/174871
dc.language.isoeng
dc.relation.ispartofLetters in Applied Microbiology
dc.relation.ispartofsjr0,616
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subjectbovine mastitis
dc.subjectdomestic animals
dc.subjectpathogenicity
dc.subjectTrueperella pyogenes
dc.subjectvirulence factors
dc.titleVirulence markers associated with Trueperella pyogenes infections in livestock and companion animalsen
dc.typeArtigo

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