DNA barcode regions for differentiating Cattleya walkeriana and C. loddigesii

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dc.contributor.authorRivera-Jiménez, Hernando [UNESP]
dc.contributor.authorRossini, Bruno César [UNESP]
dc.contributor.authorTambarussi, Evandro Vagner [UNESP]
dc.contributor.authorVeasey, Elizabeth Ann
dc.contributor.authorIbanes, Bruna
dc.contributor.authorMarino, Celso Luis [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionUniversidade Estadual do Centro-Oeste
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2022-04-29T23:53:04Z
dc.date.available2022-04-29T23:53:04Z
dc.date.issued2017-01-01
dc.description.abstractGrowers appreciate Cattleya walkeriana and C. loddigesii due to striking shape and rarity. Thus, this study aimed to evaluate the feasibility of DNA barcode regions, namely ITS1, ITS2 and rpoC1, to discriminate between C. walkeriana and C. loddigesii species. DNA barcode regions were successfully amplified using primers designed to amplify plants. We also included sequences from public databases in order to test if these regions were able to discriminate C. walkeriana and C. loddigesii from other Cattleya species. These regions, and their combinations, demonstrated that the ITS1+ITS2 had the highest average interspecific distance (11.1%), followed by rpoC1 (1.06%). For species discrimination, ITS1+ITS2 provided the best results. The combined data set of ITS1+ITS2+rpoC1 also discriminated both species, but did not result in higher rates of discrimination. These results indicate that ITS region is the best option for molecular identification of these two species and from some other species of this genus.en
dc.description.affiliationDepartamento de Genética Instituto de Biociências Universidade Estadual Paulista
dc.description.affiliationInstituto de Biotecnologia Universidade Estadual Paulista
dc.description.affiliationUniversidade Estadual do Centro-Oeste, PR-153, Km 7
dc.description.affiliationFaculdade de Ciências Agronômicas Universidade Estadual Paulista, Rua José Barbosa de Barros, 1780
dc.description.affiliationEscola Superior de Agricultura “Luiz de Queiroz” Universidade de São Paulo
dc.description.affiliationUnespDepartamento de Genética Instituto de Biociências Universidade Estadual Paulista
dc.description.affiliationUnespInstituto de Biotecnologia Universidade Estadual Paulista
dc.description.affiliationUnespFaculdade de Ciências Agronômicas Universidade Estadual Paulista, Rua José Barbosa de Barros, 1780
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipUniversidade de São Paulo
dc.format.extent45-52
dc.identifierhttp://dx.doi.org/10.4025/actascibiolsci.v39i1.33024
dc.identifier.citationActa Scientiarum - Biological Sciences, v. 39, n. 1, p. 45-52, 2017.
dc.identifier.doi10.4025/actascibiolsci.v39i1.33024
dc.identifier.issn1807-863X
dc.identifier.issn1679-9283
dc.identifier.scopus2-s2.0-85019400893
dc.identifier.urihttp://hdl.handle.net/11449/232598
dc.language.isoeng
dc.relation.ispartofActa Scientiarum - Biological Sciences
dc.sourceScopus
dc.subjectGenetic variation
dc.subjectOrchid improvement
dc.subjectSpecies separation
dc.titleDNA barcode regions for differentiating Cattleya walkeriana and C. loddigesiien
dc.titleRegiões de DNA barcode para diferenciar Cattleya walkeriana e C. loddigesiipt
dc.typeArtigo

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