Reproductive Development of Male Rats Exposed In Utero to Stress and/or Sertraline

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dc.contributor.authorQuiarato Lozano, Ana Flávia [UNESP]
dc.contributor.authorMarques Tavares, Bruna [UNESP]
dc.contributor.authorVillela E Silva, Patrícia [UNESP]
dc.contributor.authorFranco De Barros, Jorge Willian [UNESP]
dc.contributor.authorDe Grava Kempinas, Wilma [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.date.accessioned2023-07-29T16:05:32Z
dc.date.available2023-07-29T16:05:32Z
dc.date.issued2022-12-01
dc.description.abstractDespite increased prescription of sertraline during pregnancy, little is known about its action on reproductive development. Therefore, this study aimed to investigate the impact that stress, associated or not with sertraline, causes on the reproductive development of male rats. Pregnant Wistar rats were divided into 4 groups (n = 16/group): CO - received filtered water; SE - received 20 mg/kg sertraline; ST - submitted to restraint stress and received filtered water; SS - submitted to restraint stress and received sertraline. The treatment was carried out from gestational days (GDs) 13-20. The animals were euthanized on GD 20 (n = 8/group), postnatal day (PND) 45 (n = 8/group), and PND 110 (n = 8/group). The testes and epididymis were analyzed histologically, and immunohistochemistry was performed on the testes by proliferating cell nuclear antigen (PCNA) and the Wilms tumor protein (Wt1). Sperm quality was also analyzed on PND 110. The evolution of body weight, anogenital distance (AGD), and puberty installation day were also verified. Statistical analysis: 2-way ANOVA or Kruskal-Wallis test (p ≤. 05). Fetal testes presented a large number of acidophilic cells in the sertraline-exposed groups. The SS group also showed a decrease in the nuclear volume of Leydig cells. This same group showed low expression of PCNA and Wt1, decreased weight of the testes and epididymis, lower AGD, and delayed puberty installation. The adulthood groups exposed to sertraline presented alterations in sperm morphology and motility. The results demonstrated that prenatal exposure to sertraline compromises the development of the rat reproductive system.en
dc.description.affiliationLaboratory of Reproductive and Developmental Biology and Toxicology Department of Structural and Functional Biology Institute of Biosciences São Paulo State University (UNESP), SP
dc.description.affiliationUnespLaboratory of Reproductive and Developmental Biology and Toxicology Department of Structural and Functional Biology Institute of Biosciences São Paulo State University (UNESP), SP
dc.format.extent189-203
dc.identifierhttp://dx.doi.org/10.1093/toxsci/kfac100
dc.identifier.citationToxicological Sciences, v. 190, n. 2, p. 189-203, 2022.
dc.identifier.doi10.1093/toxsci/kfac100
dc.identifier.issn1096-0929
dc.identifier.issn1096-6080
dc.identifier.scopus2-s2.0-85147969609
dc.identifier.urihttp://hdl.handle.net/11449/249653
dc.language.isoeng
dc.relation.ispartofToxicological Sciences
dc.sourceScopus
dc.subjectgestational stress
dc.subjectmale rats
dc.subjectreproductive system
dc.subjectsertraline
dc.titleReproductive Development of Male Rats Exposed In Utero to Stress and/or Sertralineen
dc.typeArtigo
unesp.author.orcid0000-0003-2828-7544[1]
unesp.author.orcid0000-0002-2112-5123[5]

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