Preparation and characterization of monomodal grapevine virus a capsid protein

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dc.contributor.authorSantana, Vinícius S.
dc.contributor.authorMariutti, Ricardo B.
dc.contributor.authorEberle, Raphael J.
dc.contributor.authorUllah, Anwar
dc.contributor.authorCaruso, Icaro P.
dc.contributor.authorArni, Raghuvir K. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2015-12-07T15:33:25Z
dc.date.available2015-12-07T15:33:25Z
dc.date.issued2015
dc.description.abstractGrapevine virus A (GVA), a flexible filament of approximately 800 nm in length is composed of capsid subunits that spontaneously assembles around a positive sense genomic RNA. In addition to encapsidation, plant viruses capsid proteins (CPs) participate in other processes throughout infection and GVA CP is involved in cell-to-cell translocation of the virus. A protocol was developed to obtain low-molecular weight GVA-CP that is not prone to aggregation and spontaneous assembly and this was characterized by circular dichroism and dynamic light scattering. These results indicate the suitably of GVA-CP for X-ray crystallographic and NMR studies that should lead to the elucidation of the first three-dimensional structure of a flexible filamentous virus from the Betaflexiviridae family.en
dc.description.affiliationCentro Multiusuário de Inovação Biomolecular, Departamento de Física, Universidade Estadual Paulista (UNESP), Rua Cristóvão Colombo 2265, São Jose do Rio Preto-SP, 15054-000, Brazil. arni@sjrp.unesp.br.
dc.description.affiliationUnespCentro Multiusuário de Inovação Biomolecular, Departamento de Física, Universidade Estadual Paulista (UNESP), Rua Cristóvão Colombo 2265, São Jose do Rio Preto-SP, 15054-000, Brazil. arni@sjrp.unesp.br.
dc.format.extent712-718
dc.identifierhttp://dx.doi.org/10.2174/0929866522666150625111607#sthash.HDrSpdfL.dpuf
dc.identifier.citationProtein And Peptide Letters, v. 22, n. 8, p. 712-718, 2015.
dc.identifier.doi10.2174/0929866522666150625111607#sthash.HDrSpdfL.dpuf
dc.identifier.issn1875-5305
dc.identifier.lattes9162508978945887
dc.identifier.orcid0000-0003-2460-1145
dc.identifier.pubmed26112976
dc.identifier.urihttp://hdl.handle.net/11449/131284
dc.language.isoeng
dc.relation.ispartofProtein And Peptide Letters
dc.rights.accessRightsAcesso restrito
dc.sourcePubMed
dc.titlePreparation and characterization of monomodal grapevine virus a capsid proteinen
dc.typeArtigo
unesp.author.lattes9162508978945887[6]
unesp.author.orcid0000-0003-2460-1145[6]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências Letras e Ciências Exatas, São José do Rio Pretopt

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