Cellular immune responses in mice immunized with Anisakis simplex larval antigens

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dc.contributor.authorPerteguer, M. J.
dc.contributor.authorRodero, M.
dc.contributor.authorFlores, J. M.
dc.contributor.authorDorea, RCC
dc.contributor.authorCuellar, C.
dc.contributor.institutionUniv Complutense
dc.contributor.institutionUniv Complutense Madrid
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T15:24:03Z
dc.date.available2014-05-20T15:24:03Z
dc.date.issued2001-05-01
dc.description.abstractCellular immune responses to Anisakis simplex L3 antigens were investigated in BALB/c mice injected subcutaneously with a homologous crude extract (CE). Popliteal lymph nodes (PLN) were found to be increased in size and weight after A. simplex CE footpad injection. The effects of A. simplex CE in vitro proliferation were assayed with non-fractionated PLN cells or nylon-wool purified T cells derived from pooled lymph node cells of mice subcutaneously injected with CE. Spleen cells from immunized animals (antigen alone, or larva alone, or antigen plus larva) were studied by flow cytometry. The immunization induced a high proportion of CD4 + and TCR alpha beta + T cells. The number of B cells (CD45 + and TCR alpha beta-) in pre-immunized and infected mice was lower than that observed in animals subjected to infection only. The number of CD4 + T cells increased in the infected and in the pre-immunized and infected mice. In the latter, a decrease of CD8a + T cells was noted. The greatest increase in CD8a+ and TCR alpha beta- T cells was found in mice that had been subjected to infection only. Histological analysis showed that the most prominent lesions were gastric and intestinal in animals infected orally with one larva.en
dc.description.affiliationUniv Complutense, Fac Farm, Dept Parasitol, E-28040 Madrid, Spain
dc.description.affiliationUniv Complutense Madrid, Fac Vet, Dept Pathol Anim 2, E-28040 Madrid, Spain
dc.description.affiliationUniv Estadual Paulista, Fac Ciências & Letras Assis, Dept Ciências Biol, São Paulo, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Fac Ciências & Letras Assis, Dept Ciências Biol, São Paulo, Brazil
dc.format.extent396-404
dc.identifierhttp://dx.doi.org/10.1007/s004360000362
dc.identifier.citationParasitology Research. New York: Springer-verlag, v. 87, n. 5, p. 396-404, 2001.
dc.identifier.doi10.1007/s004360000362
dc.identifier.issn0932-0113
dc.identifier.urihttp://hdl.handle.net/11449/34715
dc.identifier.wosWOS:000168622900007
dc.language.isoeng
dc.publisherSpringer
dc.relation.ispartofParasitology Research
dc.relation.ispartofjcr2.558
dc.relation.ispartofsjr0,991
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.titleCellular immune responses in mice immunized with Anisakis simplex larval antigensen
dc.typeArtigo
dcterms.licensehttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dcterms.rightsHolderSpringer
unesp.author.orcid0000-0001-7948-9889[5]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências e Letras, Assispt

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Artigos - Ciências Biológicas - FCLAS