Direct Spinal Ventral Root Repair following Avulsion: Effectiveness of a New Heterologous Fibrin Sealant on Motoneuron Survival and Regeneration

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dc.contributor.authorDe Castro, Mateus Vidigal
dc.contributor.authorBarbizan, Roberta
dc.contributor.authorFerreira, Rui Seabra [UNESP]
dc.contributor.authorBarraviera, Benedito [UNESP]
dc.contributor.authorDe Oliveira, Alexandre Leite Rodrigues
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.contributor.institutionUniversidade Federal de Viçosa (UFV)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2018-12-11T17:05:31Z
dc.date.available2018-12-11T17:05:31Z
dc.date.issued2016-01-01
dc.description.abstractAxonal injuries at the interface between central and peripheral nervous system, such as ventral root avulsion (VRA), induce important degenerative processes, mostly resulting in neuronal and motor function loss. In the present work, we have compared two different fibrin sealants, one derived from human blood and another derived from animal blood and Crotalus durissus terrificus venom, as a promising treatment for this type of injury. Lewis rats were submitted to VRA (L4-L6) and had the avulsed roots reimplanted to the surface of the spinal cord, with the aid of fibrin sealant. The spinal cords were processed to evaluate neuronal survival, synaptic stability, and glial reactivity, 4 and 12 weeks after lesion. Sciatic nerves were processed to investigate Schwann cell activity by p 75 NTR expression (4 weeks after surgery) and to count myelinated axons and morphometric evaluation (12 weeks after surgery). Walking track test was used to evaluate gait recovery, up to 12 weeks. The results indicate that both fibrin sealants are similarly efficient. However, the snake-derived fibrin glue is a potentially safer alternative for being a biological and biodegradable product which does not contain human blood derivatives. Therefore, the venom glue can be a useful tool for the scientific community due to its advantages and variety of applications.en
dc.description.affiliationDepartment of Structural and Functional Biology Institute of Biology University of Campinas
dc.description.affiliationSchool of Medicine at Mucuri (FAMMUC) Federal University of Jequitinhonha and Mucuri Valleys (UFVJM)
dc.description.affiliationDepartment of Tropical Diseases Botucatu Medical School São Paulo State University (UNESP)
dc.description.affiliationCenter for the Study of Venoms and Venomous Animals (CEVAP) São Paulo State University (UNESP)
dc.description.affiliationUnespDepartment of Tropical Diseases Botucatu Medical School São Paulo State University (UNESP)
dc.description.affiliationUnespCenter for the Study of Venoms and Venomous Animals (CEVAP) São Paulo State University (UNESP)
dc.identifierhttp://dx.doi.org/10.1155/2016/2932784
dc.identifier.citationNeural Plasticity, v. 2016.
dc.identifier.doi10.1155/2016/2932784
dc.identifier.file2-s2.0-84985960730.pdf
dc.identifier.issn1687-5443
dc.identifier.issn2090-5904
dc.identifier.scopus2-s2.0-84985960730
dc.identifier.urihttp://hdl.handle.net/11449/173446
dc.language.isoeng
dc.relation.ispartofNeural Plasticity
dc.relation.ispartofsjr1,348
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.titleDirect Spinal Ventral Root Repair following Avulsion: Effectiveness of a New Heterologous Fibrin Sealant on Motoneuron Survival and Regenerationen
dc.typeArtigo
unesp.author.lattes6840524602748457[4]
unesp.author.orcid0000-0002-9855-5594[4]
unesp.author.orcid0000-0003-4224-4575[5]

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Artigos - Doenças Tropicais e Diagnósticos por Imagem - FMB
Botucatu - CEVAP - Centro de Estudos de Venenos e Animais Peçonhentos