Transposon display supports transpositional activity of P elements in species of the saltans group of Drosophila

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dc.contributor.authorDe Setta, Nathalia [UNESP]
dc.contributor.authorCosta, Ana Paula Pimentel
dc.contributor.authorLopes, Fabrício Ramon [UNESP]
dc.contributor.authorVan Sluys, Marie-Anne
dc.contributor.authorCarareto, Cláudia Márcia Aparecida [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2014-05-27T11:22:26Z
dc.date.available2014-05-27T11:22:26Z
dc.date.issued2007-04-01
dc.description.abstractMobilization of two P element subfamilies (canonical and O-type) from Drosophila sturtevanti and D. saltans was evaluated for copy number and transposition activity using the transposon display (TD) technique. Pairwise distances between strains regarding the insertion polymorphism profile were estimated. Amplification of the P element based on copy number estimates was highly variable among the strains (D. sturtevanti, canonical 20.11, O-type 9.00; D. saltans, canonical 16.4, O-type 12.60 insertions, on average). The larger values obtained by TD compared to our previous data by Southern blotting support the higher sensitivity of TD over Southern analysis for estimating transposable element copy numbers. The higher numbers of the canonical P element and the greater divergence in its distribution within the genome of D. sturtevanti (24.8%) compared to the O-type (16.7%), as well as the greater divergence in the distribution of the canonical P element, between the D. sturtevanti (24.8%) and the D. saltans (18.3%) strains, suggest that the canonical element occupies more sites within the D. sturtevanti genome, most probably due to recent transposition activity. These data corroborate the hypothesis that the O-type is the oldest subfamily of P elements in the saltans group and suggest that the canonical P element is or has been transpositionally active until more recently in D. sturtevanti. © Indian Academy of Sciences.en
dc.description.affiliationUniversidade Estadual Paulista Departamento de Biologia, S. José do Rio Preto, SP
dc.description.affiliationUniversidade de São Paulo Departamento de Botânica, São Paulo, SP
dc.description.affiliationUnespUniversidade Estadual Paulista Departamento de Biologia, S. José do Rio Preto, SP
dc.format.extent37-43
dc.identifierhttp://dx.doi.org/10.1007/s12041-007-0005-z
dc.identifier.citationJournal of Genetics, v. 86, n. 1, p. 37-43, 2007.
dc.identifier.doi10.1007/s12041-007-0005-z
dc.identifier.issn0022-1333
dc.identifier.lattes3425772998319216
dc.identifier.orcid0000-0002-0298-1354
dc.identifier.scopus2-s2.0-34547445660
dc.identifier.urihttp://hdl.handle.net/11449/69589
dc.identifier.wosWOS:000248375900005
dc.language.isoeng
dc.relation.ispartofJournal of Genetics
dc.relation.ispartofjcr0.672
dc.relation.ispartofsjr0,357
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subjectCanonical P element
dc.subjectDrosophila saltans
dc.subjectDrosophila sturtevanti
dc.subjectO-type P element
dc.subjectTransposon display
dc.subjectTransposon mobilization
dc.subjectprimer DNA
dc.subjectanimal
dc.subjectclassification
dc.subjectCosta Rica
dc.subjectDNA fingerprinting
dc.subjectDrosophila
dc.subjectgenetic polymorphism
dc.subjectgenetics
dc.subjectgeography
dc.subjectMexico
dc.subjectpolymerase chain reaction
dc.subjectSouth America
dc.subjecttransposon
dc.subjectAnimals
dc.subjectDNA Fingerprinting
dc.subjectDNA Primers
dc.subjectDNA Transposable Elements
dc.subjectGeography
dc.subjectPolymerase Chain Reaction
dc.subjectPolymorphism, Genetic
dc.subjectsaltans group
dc.titleTransposon display supports transpositional activity of P elements in species of the saltans group of Drosophilaen
dc.typeArtigo
dcterms.licensehttp://www.springer.com/open+access/authors+rights
unesp.author.lattes3425772998319216[5]
unesp.author.orcid0000-0002-0298-1354[5]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências Letras e Ciências Exatas, São José do Rio Pretopt

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