In vitro embryos production after oocytes treatment with forskolin

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dc.contributor.authorPaschoal, Daniela Martins [UNESP]
dc.contributor.authorMaziero, Rosiára Rosária Dias [UNESP]
dc.contributor.authorSudano, Mateus José [UNESP]
dc.contributor.authorGuastali, Midyan Daroz [UNESP]
dc.contributor.authorVergara, Luis Eduardo [UNESP]
dc.contributor.authorCrocomo, Letícia Ferrari [UNESP]
dc.contributor.authorLima-Neto, João Ferreira de [UNESP]
dc.contributor.authorMagalhães, Luis Carlos Oña [UNESP]
dc.contributor.authorMonteiro, Bianca Andriolo [UNESP]
dc.contributor.authorRascado, Tatiana da Silva [UNESP]
dc.contributor.authorMartins, Alício [UNESP]
dc.contributor.authorLeal, Claudia Lima Verde
dc.contributor.authorLandim-Alvarenga, Fernanda da Cruz [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2015-12-07T15:36:13Z
dc.date.available2015-12-07T15:36:13Z
dc.date.issued2015-02-24
dc.description.abstractSummary The inhibition of nuclear maturation allows time for the oocyte to accumulate molecules that are important for embryonic development. Thus, the objective of this work was to evaluate the effect of blocking oocyte meiosis with the addition of forskolin, an efficient inhibitor of nuclear maturation, in in vitro maturation (IVM) medium. Forskolin was added to the IVM medium for 6 h at concentrations of 0.1 mM, 0.05 mM or 0.025 mM, then the oocytes were allowed to mature in drug-free medium for 18 h. The oocytes were assessed for the stage of nuclear maturation, the activity and distribution of mitochondria, oocyte ultrastructure, the number of viable cells and the apoptosis rate. After forskolin treatment, the oocytes were fertilized in vitro and cultured for 7 days. On day 7, the blastocyst rate, the ultrastructure, the number of intact cells and the apoptosis rate of the blastocysts were measured. No differences were observed for the stage of nuclear maturation of the oocyte, the mitochondrial activity and distribution, the blastocyst rate or total number of intact cells. However, a higher rate of apoptosis was observed in the blastocysts produced from oocytes blocked for 6 h with the higher concentration of forskolin (P < 0.05). We conclude that all the experimental groups reached the MII stage after the addition of forskolin and that the highest concentration of forskolin caused cellular degeneration without harming embryo production on the 7th day.en
dc.description.affiliationSão Paulo State University (UNESP),School of Veterinary Medicine and Animal Science (FMVZ),Department of Animal Reproduction and Veterinary Radiology,Rubião Jr. s/n,Botucatu/SP,Brazil.
dc.description.affiliationDepartment of Animal Reproduction and Veterinary Radiology,School of Veterinary Medicine and Animal Science (FMVZ),São Paulo State University (UNESP),Botucatu,SP,Brazil.
dc.description.affiliationDepartment of Clinical Surgery and Animal Reproduction,School of Veterinary Medicine (FMVA),São Paulo State University (UNESP),Araçatuba,SP,Brazil.
dc.description.affiliationDepartment of Basic Science,School of Animal Science and Food Engineering (FZEA),São Paulo University (USP),Pirassununga/SP,Brazil.
dc.description.affiliationUnespSão Paulo State University (UNESP),School of Veterinary Medicine and Animal Science (FMVZ),Department of Animal Reproduction and Veterinary Radiology,Rubião Jr. s/n,Botucatu/SP,Brazil.
dc.description.affiliationUnespDepartment of Animal Reproduction and Veterinary Radiology,School of Veterinary Medicine and Animal Science (FMVZ),São Paulo State University (UNESP),Botucatu,SP,Brazil.
dc.description.affiliationUnespDepartment of Clinical Surgery and Animal Reproduction,School of Veterinary Medicine (FMVA),São Paulo State University (UNESP),Araçatuba,SP,Brazil.
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 2010/50410–2
dc.description.sponsorshipIdFAPESP: 2011/08926–4
dc.format.extent1-11
dc.identifierhttp://dx.doi.org/10.1017/S0967199414000835
dc.identifier.citationZygote (cambridge, England), p. 1-11, 2015.
dc.identifier.doi10.1017/S0967199414000835
dc.identifier.issn1469-8730
dc.identifier.pubmed25707683
dc.identifier.urihttp://hdl.handle.net/11449/131483
dc.language.isoeng
dc.publisherCambridge University Press
dc.relation.ispartofZygote (cambridge, England)
dc.relation.ispartofsjr0,387
dc.rights.accessRightsAcesso restrito
dc.sourcePubMed
dc.subjectApoptosisen
dc.subjectcAMPen
dc.subjectForskolinen
dc.subjectIVMen
dc.subjectMeiosisen
dc.titleIn vitro embryos production after oocytes treatment with forskolinen
dc.typeArtigo
dcterms.rightsHolderCambridge University Press
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina Veterinária, Araçatubapt
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina Veterinária e Zootecnia, Botucatupt

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Artigos - Reprodução Animal e Radiologia Veterinária - FMVZ
Artigos - Clínica, Cirurgia e Reprodução Animal - FMVA