Lack of DNA damage induced by fluoride on mouse lymphoma and human fibroblast cells by single cell gel (comet) assay

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dc.contributor.authorRibeiro, Daniel Araki [UNESP]
dc.contributor.authorAlves de Lima, Patrícia Lepage
dc.contributor.authorMarques, Mariângela Esther Alencar [UNESP]
dc.contributor.authorSalvadori, Daisy Maria Favero [UNESP]
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-27T11:22:04Z
dc.date.available2014-05-27T11:22:04Z
dc.date.issued2006-12-01
dc.description.abstractFluoride has widely been used in Dentistry because it is a specific and effective caries prophylactic agent. However, excess fluoride may represent a hazard to human health, especially by causing injury on genetic apparatus. Genotoxicity tests constitute an important part of cancer research for risk assessment of potential carcinogens. In this study, the potential DNA damage associated with exposure to fluoride was assessed by the single cell gel (comet) assay in vitro. Mouse lymphoma and human fibroblast cells were exposed to sodium fluoride (NaF) at final concentration ranging from 7 to 100 μg/mL for 3 h at 37μC. The results pointed out that NaF in all tested concentrations did not contribute to DNA damage as depicted by the mean tail moment and tail intensity for both cellular types assessed. These findings are clinically important because they represent a valuable contribution for evaluation of the potential health risk associated with exposure to agents usually used in dental practice.en
dc.description.affiliationCenter for Genotoxin and Carcinogen Evaluation (TOXICAN) Department of Pathology State University of São Paulo, Botucatu, SP
dc.description.affiliationNúcleo de Avaliação Toxicogenética e Cancerígena (TOXICAN) Departamento de Patologia UNESP, Distrito de Rubião Jr. S/N, 18618-000 Botucatu, SP
dc.description.affiliationUnespNúcleo de Avaliação Toxicogenética e Cancerígena (TOXICAN) Departamento de Patologia UNESP, Distrito de Rubião Jr. S/N, 18618-000 Botucatu, SP
dc.format.extent91-94
dc.identifierhttp://dx.doi.org/10.1590/S0103-64402006000200001
dc.identifier.citationBrazilian Dental Journal, v. 17, n. 2, p. 91-94, 2006.
dc.identifier.doi10.1590/S0103-64402006000200001
dc.identifier.file2-s2.0-34648818965.pdf
dc.identifier.issn0103-6440
dc.identifier.issn1806-4760
dc.identifier.lattes5051118752980903
dc.identifier.lattes7528116925519142
dc.identifier.scieloS0103-64402006000200001
dc.identifier.scopus2-s2.0-34648818965
dc.identifier.urihttp://hdl.handle.net/11449/69288
dc.language.isoeng
dc.relation.ispartofBrazilian Dental Journal
dc.relation.ispartofsjr0,476
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectComet assay
dc.subjectHuman fibroblasts
dc.subjectMouse lymphoma cells
dc.subjectSodium fluoride
dc.subjectanticaries agent
dc.subjectfluoride sodium
dc.subjectanalysis of variance
dc.subjectanimal
dc.subjectcomet assay
dc.subjectDNA damage
dc.subjectdrug effect
dc.subjectfibroblast
dc.subjectgenetics
dc.subjecthuman
dc.subjectlymphoma
dc.subjectmouse
dc.subjectpathology
dc.subjectAnalysis of Variance
dc.subjectAnimals
dc.subjectCariostatic Agents
dc.subjectComet Assay
dc.subjectDNA Damage
dc.subjectFibroblasts
dc.subjectHumans
dc.subjectLymphoma
dc.subjectMice
dc.subjectSodium Fluoride
dc.titleLack of DNA damage induced by fluoride on mouse lymphoma and human fibroblast cells by single cell gel (comet) assayen
dc.typeArtigo
dcterms.licensehttp://www.scielo.br/revistas/bdj/paboutj.htm
unesp.author.lattes5051118752980903
unesp.author.lattes7528116925519142
unesp.author.orcid0000-0001-6947-5627[3]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina, Botucatupt

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