Glutaminase-free L-asparaginase production by Leucosporidium muscorum isolated from Antarctic marine-sediment

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dc.contributor.authorFreire, Rominne Karla Barros
dc.contributor.authorMendonça, Carlos Miguel Nóbrega
dc.contributor.authorFerraro, Rafael Bertelli
dc.contributor.authorMoguel, Ignacio Sánchez
dc.contributor.authorTonso, Aldo
dc.contributor.authorLourenço, Felipe Rebello
dc.contributor.authorSantos, João Henrique Picado Madalena
dc.contributor.authorSette, Lara Durães [UNESP]
dc.contributor.authorPessoa Junior, Adalberto
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2020-12-12T02:49:51Z
dc.date.available2020-12-12T02:49:51Z
dc.date.issued2020-01-01
dc.description.abstractL-asparaginase (ASNase) is an essential drug in the treatment of acute lymphoblastic leukemia (ALL). Commercial bacterial ASNases increase patient survival, but the consequent immunological reactions remain a challenge. Yeasts ASNase is closer to human congeners and could lead to lower side effects. Among 134 yeast strains isolated from marine-sediments in King George Island, Antarctica, nine were L-asparaginase producing yeasts and glutaminase-free. Leucosporidium muscorum CRM 1648 yielded the highest ASNase activity (490.41 U.L−1) and volumetric productivity (5.12 U.L−1 h−1). Sucrose, yeast extract and proline were the best carbon and nitrogen sources to support growth and ASNase production. A full factorial design analysis pointed the optimum media condition for yeast growth and ASNase yield: 20 g L−1 sucrose, 15 g L−1 yeast extract and 20 g L−1 proline, which resulted in 4582.5 U L−1 and 63.64 U L−1 h−1 of ASNase and volumetric productivity, respectively. Analysis of temperature, pH, inoculum and addition of seawater indicated the best condition for ASNase production by this yeast: 12–15 °C, pH 5.5–6.5 and seawater >25% (v/v). Inoculum concentration seems not to interfere. This work is pioneer on the production of ASNase by cold-adapted yeasts, highlighting the potential of these microbial resources as a source of glutaminase-free L-asparaginase for commercial purposes.en
dc.description.affiliationDepartment of Biochemical and Pharmaceutical Technology School of Pharmaceutical Sciences University of Sao Paulo
dc.description.affiliationDepartment of Chemical Engineering Polytechnic School University of Sao Paulo
dc.description.affiliationDepartment of Pharmacy School of Pharmaceutical Sciences University of Sao Paulo
dc.description.affiliationDepartment of General and Applied Biology Institute of Biosciences State University Julio de Mesquita Filho (UNESP)
dc.description.affiliationUnespDepartment of General and Applied Biology Institute of Biosciences State University Julio de Mesquita Filho (UNESP)
dc.identifierhttp://dx.doi.org/10.1080/10826068.2020.1815053
dc.identifier.citationPreparative Biochemistry and Biotechnology.
dc.identifier.doi10.1080/10826068.2020.1815053
dc.identifier.issn1532-2297
dc.identifier.issn1082-6068
dc.identifier.scopus2-s2.0-85090947334
dc.identifier.urihttp://hdl.handle.net/11449/202103
dc.language.isoeng
dc.relation.ispartofPreparative Biochemistry and Biotechnology
dc.sourceScopus
dc.subjectCold-adapted yeast
dc.subjectenzyme production
dc.subjectglutaminase-free asparaginase
dc.subjectL-asparaginase
dc.subjectLeucosporidium muscorum
dc.titleGlutaminase-free L-asparaginase production by Leucosporidium muscorum isolated from Antarctic marine-sedimenten
dc.typeArtigo
unesp.author.orcid0000-0002-5268-8690[9]

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