The role of melatonin on miRNAs modulation in triple-negative breast cancer cells

Página do item simplificado
dc.contributor.authorFerreira, Livia C. [UNESP]
dc.contributor.authorOrso, Francesca
dc.contributor.authorDettori, Daniela
dc.contributor.authorLacerda, Jessica Z. [UNESP]
dc.contributor.authorBorin, Thaiz F.
dc.contributor.authorTaverna, Daniela
dc.contributor.authorZuccari, Debora A. P. C. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniv Torino
dc.contributor.institutionAugusta Univ
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2020-12-10T19:59:34Z
dc.date.available2020-12-10T19:59:34Z
dc.date.issued2020-02-03
dc.description.abstractMelatonin, a hormone secreted by pineal gland, exerts antimetastatic effects by reducing tumor cell proliferation, migration and invasion. MicroRNAs (miRNAs) are small, non-coding RNAs that play a crucial role in regulation of gene expression and biological processes of the cells. Herein, we search for a link between the tumor/metastatic-suppressive actions of melatonin and miRNA expression in triple-negative breast cancer cells. We demonstrated that melatonin exerts its anti-tumor actions by reducing proliferation, migration and c-Myc expression of triple negative breast cancer cells. By using Taqman-based assays, we analyzed the expression levels of a set of miRNAs following melatonin treatment of triple negative breast cancer cells and we identified 17 differentially expressed miRNAs, 6 down-regulated and 11 up-regulated. We focused on the anti-metastatic miR-148b and the oncogenic miR-210 both up-regulated by melatonin treatment and studied the effect of their modulation on melatonin-mediated impairment of tumor progression. Surprisingly, when miR-148b or miR-210 were depleted in triple-negative breast cancer cells, using a specific miR-148b sponge or anti-miR-210, melatonin effects on migration inhibition and c-myc downregulation were still visible suggesting that the increase of miR-148b and miR-210 expression observed following melatonin treatment was not required for the efficacy of melatonin action. Nevertheless, ours results suggest that melatonin exhibit a compound for metastatic trait inhibition, especially in MDA-MB-231 breast cancer cells even if a direct link between modulation of expression of certain proteins or miRNAs and melatonin effects has still to be established.en
dc.description.affiliationUniv Estadual Paulista, Dept Biol, Sao Jose Do Rio Preto, SP, Brazil
dc.description.affiliationUniv Torino, Mol Biotechnol Ctr MBC, Dept Mol Biotechnol & Hlth Sci, Turin, Italy
dc.description.affiliationAugusta Univ, Dept Biochem & Mol Biol, Tumor Angiogenesis Lab, Augusta, GA USA
dc.description.affiliationFac Med Sao Jose do Rio Preto, Dept Mol Biol, Sao Jose Do Rio Preto, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Dept Biol, Sao Jose Do Rio Preto, SP, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 2015/04780-6
dc.format.extent15
dc.identifierhttp://dx.doi.org/10.1371/journal.pone.0228062
dc.identifier.citationPlos One. San Francisco: Public Library Science, v. 15, n. 2, 15 p., 2020.
dc.identifier.doi10.1371/journal.pone.0228062
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/11449/196896
dc.identifier.wosWOS:000534615800018
dc.language.isoeng
dc.publisherPublic Library Science
dc.relation.ispartofPlos One
dc.sourceWeb of Science
dc.titleThe role of melatonin on miRNAs modulation in triple-negative breast cancer cellsen
dc.typeArtigo
dcterms.rightsHolderPublic Library Science

Arquivos

Coleções

Artigos - Biologia - IBILCE