Establishment and characterization of a cell line (RBME-6) of Rhipicephalus (Boophilus) microplus from Brazil

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dc.contributor.authorLima-Duarte, Leidiane
dc.contributor.authorCamargo, Jaqueline Valéria [UNESP]
dc.contributor.authorCastro-Santiago, Ana Carolina
dc.contributor.authorMachado, Rosangela Zacarias [UNESP]
dc.contributor.authorAndré, Marcos Rogério [UNESP]
dc.contributor.authorCabral-de-Mello, Diogo Cavalcanti [UNESP]
dc.contributor.authorCamargo-Mathias, Maria Izabel [UNESP]
dc.contributor.authorIkeda, Priscila [UNESP]
dc.contributor.authorAnholeto, Luís Adriano [UNESP]
dc.contributor.authorPereira, Melissa Carolina [UNESP]
dc.contributor.authorda Costa, Alvimar José [UNESP]
dc.contributor.authorBarros-Battesti, Darci Moraes [UNESP]
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.date.accessioned2022-05-01T06:02:08Z
dc.date.available2022-05-01T06:02:08Z
dc.date.issued2021-09-01
dc.description.abstractTick cell lines have already proved to be a useful tool for obtaining more information about possible vector species and the factors governing their ability to transmit a pathogen. Here, we established and characterized a cell line (RBME-6) derived from embryos of Rhipicephalus microplus from Brazil. Primary tick cell cultures were prepared in L-15B medium supplemented with 20% fetal bovine serum and 10% tryptose phosphate broth. The cell monolayers were subcultured when they reached a density of approximately 8 × 10 5 cells/mL (95% viability). Only after the sixth subculture were cells thawed from storage in liquid nitrogen successfully. Cytological analyses were performed using live phase contrast microscopy and cytocentrifuge smears stained with Giemsa, while periodic acid-Schiff and bromophenol blue staining techniques were used to detect total polysaccharides and total protein, respectively. No DNA from Anaplasma spp., Anaplasma marginale, Babesia spp., Bartonella spp., Coxiella spp., Ehrlichia canis, Rickettsia spp. or Mycoplasma spp. was detected in the cells through PCR assays. In addition, we performed chromosomal characterization of the tick cell line and confirmed the R. microplus origin of the cell line through conventional PCR and sequencing of a fragment of the mitochondrial 16S rRNA gene. In conclusion, we established and characterized a new cell line from a Brazilian population of R. microplus, which may form a useful tool for studying several aspects of ticks and tick-borne pathogens.en
dc.description.affiliationDepartment of Preventive Veterinary Medicine and Animal Science School of Veterinary Medicine University of São Paulo
dc.description.affiliationDepartment of Pathology Reproduction and One Health School of Agricultural and Veterinary Sciences São Paulo State University “Julio de Mesquita Filho” (UNESP)
dc.description.affiliationDepartment of General and Applied Biology Institute of Biosciences São Paulo State University “Julio de Mesquita Filho” (UNESP)
dc.description.affiliationUnespDepartment of Pathology Reproduction and One Health School of Agricultural and Veterinary Sciences São Paulo State University “Julio de Mesquita Filho” (UNESP)
dc.description.affiliationUnespDepartment of General and Applied Biology Institute of Biosciences São Paulo State University “Julio de Mesquita Filho” (UNESP)
dc.identifierhttp://dx.doi.org/10.1016/j.ttbdis.2021.101770
dc.identifier.citationTicks and Tick-borne Diseases, v. 12, n. 5, 2021.
dc.identifier.doi10.1016/j.ttbdis.2021.101770
dc.identifier.issn1877-9603
dc.identifier.issn1877-959X
dc.identifier.scopus2-s2.0-85109023391
dc.identifier.urihttp://hdl.handle.net/11449/233216
dc.language.isoeng
dc.relation.ispartofTicks and Tick-borne Diseases
dc.sourceScopus
dc.subjectBrazil
dc.subjectCattle tick
dc.subjectCell line
dc.subjectChromosome
dc.titleEstablishment and characterization of a cell line (RBME-6) of Rhipicephalus (Boophilus) microplus from Brazilen
dc.typeArtigo
unesp.author.orcid0000-0001-5522-9185[1]
unesp.author.orcid0000-0002-0002-7743[8]

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