Effects of Brazilian and Bulgarian propolis on bactericidal activity of macrophages against Salmonella Typhimurium

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dc.contributor.authorOrsi, Ricardo de Oliveira [UNESP]
dc.contributor.authorSforcin, J. M.
dc.contributor.authorFunari, SRC
dc.contributor.authorBankova, N.
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionBulgarian Acad Sci
dc.date.accessioned2014-05-20T13:50:35Z
dc.date.available2014-05-20T13:50:35Z
dc.date.issued2005-02-01
dc.description.abstractPropolis has been used in folk medicine since ancient times due to its, many biological properties: such as antimicrobial. antinflammatory, antioxidant, immunomodulatory activities, among others. 'Macrophages play an important role in the early phase of Salmonella infection. In this work, macrophages were prestimated with Brazilian or Bulgarian propolis and subsequently challenged with Salmonella Typhimurium at different macrophage/bacteria ratio. After 60 min of incubation. cells were harvested with Triton-X to lyse the macrophages. To assess the bactericidal activity. The number of colony-forming units (CFU) of S. typhimurium was determined by plating 0.1 mL in 'Mueller Hinton agar. After 24 h. CFU were counted. and the percentage of bactericidal activity was obtained. Propolis from Brazil and Bulgaria enhanced the bactericidal activity of macrophages, depending on its concentration. Brazilian propolis seemed to be more efficient than that from Bulgaria. because of their different chemical composition. In Bulgaria, bees collect the material mainly from the bud exudate of poplar tree, while in Brazil, Baccharis dracunculifolia DC. was shown to be the main propolis source. Our data also showed that the increased bactericidal activity of macrophages involved the participation of oxygen (H2O2) and nitrogen (NO) intermediate metabolites. (C) 2004 Elsevier B.V. All rights reserved.en
dc.description.affiliationUniv Estadual Paulista Julio Mesquita Filho, Dept Microbiol & Immunol, BR-18618000 Botucatu, SP, Brazil
dc.description.affiliationUniv Estadual Paulista Julio Mesquita Filho, Biosci Inst, BR-18618000 Botucatu, SP, Brazil
dc.description.affiliationUniv Estadual Paulista Julio Mesquita Filho, Sch Vet Med & Anim Husb, Dept Prod & Anim Explorat, BR-18618000 Botucatu, SP, Brazil
dc.description.affiliationBulgarian Acad Sci, Ctr Phytochem, Inst Organ Chem, BU-1113 Sofia, Bulgaria
dc.description.affiliationUnespUniv Estadual Paulista Julio Mesquita Filho, Dept Microbiol & Immunol, BR-18618000 Botucatu, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista Julio Mesquita Filho, Biosci Inst, BR-18618000 Botucatu, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista Julio Mesquita Filho, Sch Vet Med & Anim Husb, Dept Prod & Anim Explorat, BR-18618000 Botucatu, SP, Brazil
dc.format.extent359-368
dc.identifierhttp://dx.doi.org/10.1016/j.intimp.2004.10.003
dc.identifier.citationInternational Immunopharmacology. Amsterdam: Elsevier B.V., v. 5, n. 2, p. 359-368, 2005.
dc.identifier.doi10.1016/j.intimp.2004.10.003
dc.identifier.issn1567-5769
dc.identifier.urihttp://hdl.handle.net/11449/18060
dc.identifier.wosWOS:000226697600012
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofInternational Immunopharmacology
dc.relation.ispartofjcr3.118
dc.relation.ispartofsjr1,051
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectpropolispt
dc.subjectmacrophagespt
dc.subjecthydrogen peroxidept
dc.subjectnitric oxidept
dc.subjectBrazilpt
dc.subjectBulgariapt
dc.titleEffects of Brazilian and Bulgarian propolis on bactericidal activity of macrophages against Salmonella Typhimuriumen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.
unesp.author.orcid0000-0001-8099-8277[1]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatupt
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina Veterinária e Zootecnia, Botucatupt

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