Hydrogen peroxide enhances the efficacy of photodynamic therapy against Candida albicans biofilms

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dc.contributor.authorViana de Sousa, Tábata [UNESP]
dc.contributor.authorCarolina Jordão, Cláudia [UNESP]
dc.contributor.authorAugusto Abreu-Pereira, César [UNESP]
dc.contributor.authorGorayb Pereira, Ana Luiza [UNESP]
dc.contributor.authorBarbugli, Paula Aboud [UNESP]
dc.contributor.authorKlein, Marlise Inêz
dc.contributor.authorPavarina, Ana Claudia [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.date.accessioned2023-07-29T13:46:08Z
dc.date.available2023-07-29T13:46:08Z
dc.date.issued2023-01-01
dc.description.abstractThe present study aimed to evaluate the effectiveness of hydrogen peroxide (H2O2) combined with antimicrobial photodynamic therapy (aPDT) on biofilms formed by Candida albicans strains which are either susceptible to or resistant to fluconazole. Biofilms were grown and treated with H2O2, followed by the application of Photodithazine® (P) and red light-emitting diode (LED) (L) either separately or combined (n = 12). After the treatment, biofilms were evaluated by estimating colony-forming unit ml−1, extracellular matrix components [water -soluble and -insoluble polysaccharides, proteins, extracellular DNA (eDNA)], biomass (total and insoluble dry-weight), and protein concentration. Biofilms formed by both strains presented a significant reduction in cell viability, biomass, extracellular matrix components (both types of polysaccharides, eDNA), and proteins (in the soluble and insoluble portion of biofilms) compared to the control. Microscopy images of the biofilms after treatments showed disarticulation of the matrix and scattered fungal cells. The application of H2O2 can disturb the organization of the extracellular matrix, and its association with aPDT potentiated the effect of the treatment.en
dc.description.affiliationDepartment of Dental Materials and Prosthodontics School of Dentistry at Araraquara Univ Estadual Paulista - UNESP, SP
dc.description.affiliationDepartment of Oral Diagnosis Piracicaba Dental School State University of Campinas (UNICAMP)
dc.description.affiliationUnespDepartment of Dental Materials and Prosthodontics School of Dentistry at Araraquara Univ Estadual Paulista - UNESP, SP
dc.format.extent94-109
dc.identifierhttp://dx.doi.org/10.1080/08927014.2023.2189011
dc.identifier.citationBiofouling, v. 39, n. 1, p. 94-109, 2023.
dc.identifier.doi10.1080/08927014.2023.2189011
dc.identifier.issn1029-2454
dc.identifier.issn0892-7014
dc.identifier.scopus2-s2.0-85149991401
dc.identifier.urihttp://hdl.handle.net/11449/248514
dc.language.isoeng
dc.relation.ispartofBiofouling
dc.sourceScopus
dc.subjectCandida albicans
dc.subjecthydrogen peroxide
dc.subjectPhotochemotherapy
dc.titleHydrogen peroxide enhances the efficacy of photodynamic therapy against Candida albicans biofilmsen
dc.typeArtigo
unesp.author.orcid0000-0002-7916-1557[6]
unesp.author.orcid0000-0002-9231-1994[7]

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