Anti-poliovirus activity of Baccharis dracunculifolia and propolis by cell viability determination and real-time PCR

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dc.contributor.authorBufalo, M. C. [UNESP]
dc.contributor.authorFigueiredo, A. S. [UNESP]
dc.contributor.authorde Sousa, J. P. B.
dc.contributor.authorCandeias, J. M. G. [UNESP]
dc.contributor.authorBastos, J. K.
dc.contributor.authorSforcin, J. M. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2014-05-20T13:51:00Z
dc.date.available2014-05-20T13:51:00Z
dc.date.issued2009-11-01
dc.description.abstractAims:The aim of this work was to evaluate the antiviral activities of Baccharis dracunculifolia (extract and essential oil), propolis and some isolated compounds (caffeic and cinnamic acids) against poliovirus type 1 (PV1) replication in HEp-2 cells.Method:Three different protocols (pre-, simultaneous and post-treatments) were used to verify the effect of addition time of the variables on PV1 replication by crystal violet method and relative viral RNA quantification by real-time PCR for analysing in which step of virus replication the variables could interfere.Conclusions:Data revealed that the B. dracunculifolia showed the best antiviral activity percentage in the simultaneous treatment, as well as lower relative viral quantification by real-time PCR. Variables might block partially the viral entry within cells, affect the steps of viral cycle replication into cells, or lead to RNA degradation before the virus entry into cells or after their release to the supernatant.Significance and Impact of the Study:Baccharis dracunculifolia is the most important botanical source of the south-eastern Brazilian propolis, and its potential for the development of new phytotherapeutic medicines has been investigated. Propolis is commonly used for its antimicrobial and immunomodulatory activities. Nevertheless, B. dracunculifolia and propolis effects on PV1 have not been investigated yet.en
dc.description.affiliationUNESP, Dept Microbiol & Immunol, Biosci Inst, BR-18618000 Botucatu, SP, Brazil
dc.description.affiliationUSP, Sch Pharmaceut Sci Ribeirao Preto, Dept Pharmaceut Sci, Lab Pharmacognosy, Ribeirao Preto, SP, Brazil
dc.description.affiliationUnespUNESP, Dept Microbiol & Immunol, Biosci Inst, BR-18618000 Botucatu, SP, Brazil
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.format.extent1669-1680
dc.identifierhttp://dx.doi.org/10.1111/j.1365-2672.2009.04354.x
dc.identifier.citationJournal of Applied Microbiology. Malden: Wiley-blackwell Publishing, Inc, v. 107, n. 5, p. 1669-1680, 2009.
dc.identifier.doi10.1111/j.1365-2672.2009.04354.x
dc.identifier.issn1364-5072
dc.identifier.orcid0000-0003-1515-702X
dc.identifier.urihttp://hdl.handle.net/11449/18206
dc.identifier.wosWOS:000270658100029
dc.language.isoeng
dc.publisherWiley-Blackwell Publishing, Inc
dc.relation.ispartofJournal of Applied Microbiology
dc.relation.ispartofjcr2.160
dc.relation.ispartofsjr0,795
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectantiviral actionen
dc.subjectBaccharis dracunculifoliaen
dc.subjectpoliovirusen
dc.subjectpropolisen
dc.subjectreal-time PCRen
dc.titleAnti-poliovirus activity of Baccharis dracunculifolia and propolis by cell viability determination and real-time PCRen
dc.typeArtigo
dcterms.licensehttp://www.nhm.ac.uk/hosted_sites/acarology/saas/saa/aim.html
dcterms.rightsHolderWiley-blackwell Publishing, Inc
unesp.author.orcid0000-0003-1515-702X[4]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatupt

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Artigos - Microbiologia e Imunologia - IBB