Gene mutations in esophageal mucosa of chagas disease patients

Nenhuma Miniatura disponível

Data

2009-04-01

Orientador

Coorientador

Pós-graduação

Curso de graduação

Título da Revista

ISSN da Revista

Título de Volume

Editor

Tipo

Artigo

Direito de acesso

Resumo

Background: Chagas disease patients with longstanding megaesophagus have a significantly increased risk for esophageal carcinoma. Materials and Methods: PCR- SSCP analysis and DNA sequencing of esophageal mucosa from Chagas disease patients (with or without megaesophagus) in the exons of the TPS3, CDKN2A and FHIT genes were performed. Results: SSCP analysis showed a mobility shift in 2/20 patients with grade TV megaesophagus (CDKN2A exon 1 and FHIT exon 7) and in 1/10 patients without megaesophagus (TP53 exons 5 and 8). However, DNA sequencing indicated a silent mutation in exon 7 of the FHIT gene (GCT → GCC) in codon 88 in only one case of megaesophagus. Conclusion: Gene mutations are rare events in the exons investigated in esophageal mucosa of Chagas disease patients and further investigations are important to clarify the possible involvement of this silent mutation in exon 7 (FHIT gene) in the advanced grades of megaesophagus and esophageal carcinogenesis.

Descrição

Idioma

Inglês

Como citar

Anticancer Research, v. 29, n. 4, p. 1243-1248, 2009.

Itens relacionados

Financiadores