Tumor-infiltrating CD4+ and CD8+ lymphocytes and macrophages are associated with prognostic factors in triple-negative canine mammary complex type carcinoma

Página do item simplificado
dc.contributor.authorFranzoni, Mayara Simão [UNESP]
dc.contributor.authorBrandi, Andressa [UNESP]
dc.contributor.authorde Oliveira Matos Prado, Jane Karlla
dc.contributor.authorElias, Fabiana
dc.contributor.authorDalmolin, Fabíola
dc.contributor.authorde Faria Lainetti, Patricia [UNESP]
dc.contributor.authorPrado, Maria Carolina Mangini [UNESP]
dc.contributor.authorLeis-Filho, Antonio Fernando [UNESP]
dc.contributor.authorFonseca-Alves, Carlos Eduardo [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionFederal University of the Fronteira Sul
dc.contributor.institutionUniversidade Paulista – UNIP
dc.date.accessioned2019-10-06T16:42:49Z
dc.date.available2019-10-06T16:42:49Z
dc.date.issued2019-10-01
dc.description.abstractThis study aimed to evaluate the association of CD3+, CD4+, and CD8+ T cells and tumor-infiltrating macrophages (TIMs) with the clinical parameters of female dogs harboring mammary gland tumors. Thirty female dogs affected with mammary carcinomas were used, and all tumors were histologically classified as complex carcinoma and were triple-negative phenotype determined by immunohistochemistry. Freshly frozen sections were used to determine CD3+, CD4+ and CD8+ T cells by immunohistochemistry, and TIMs were determined by immunofluorescence assays. Ten out of the 30 dogs showed lymph node metastasis at diagnosis. Fifteen dogs had a tumor of grade I (15/30), nine (9/30) had a tumor of grade II and six (6/30) had a tumor of grade III. The mean overall survival was 680.5 days (± 200.4). Dogs with sentinel lymph node positivity (10/30) (P =.0035) and dogs that developed metastasis (P =.0001) showed a shorter survival time. In addition, dogs with a high level of inflammatory infiltrate in tumor tissues presented a shorter survival time (P =.0001) than that of other dogs. Dogs with tumors containing higher numbers of CD3+ T cells (P =.001), CD4+ T cells (P =.001), or TIM cells (P <.0001) showed a shorter survival time than that of other dogs. Our results suggested that characteristics of immune cell infiltrates, including CD3+ T cells, CD4+ T cells, and TIMs, can be used as potential prognostic indicators for predicting clinical outcomes in dogs with mammary gland tumors, particularly tumors with a complex histological subtype and triple-negative phenotype.en
dc.description.affiliationDepartment of Veterinary Surgery and Anesthesiology School of Veterinary Medicine and Animal Science São Paulo State University – UNESP
dc.description.affiliationSuperintendencia Unidade Hospitalar Veterinaria Universitaria Federal University of the Fronteira Sul
dc.description.affiliationUniversidade Paulista – UNIP
dc.description.affiliationUnespDepartment of Veterinary Surgery and Anesthesiology School of Veterinary Medicine and Animal Science São Paulo State University – UNESP
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: #2016/21474-9
dc.format.extent29-36
dc.identifierhttp://dx.doi.org/10.1016/j.rvsc.2019.08.021
dc.identifier.citationResearch in Veterinary Science, v. 126, p. 29-36.
dc.identifier.doi10.1016/j.rvsc.2019.08.021
dc.identifier.issn1532-2661
dc.identifier.issn0034-5288
dc.identifier.scopus2-s2.0-85070586036
dc.identifier.urihttp://hdl.handle.net/11449/189503
dc.language.isoeng
dc.relation.ispartofResearch in Veterinary Science
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectCanine
dc.subjectInflammatory cells
dc.subjectLymphocytes
dc.subjectMammary gland tumors
dc.subjectMononuclear cells
dc.titleTumor-infiltrating CD4+ and CD8+ lymphocytes and macrophages are associated with prognostic factors in triple-negative canine mammary complex type carcinomaen
dc.typeArtigo

Arquivos

Coleções

Artigos - Cirurgia e Anestesiologia Veterinária - FMVZ