Putative pyrophosphate phosphofructose 1-kinase genes identified in sugarcane may be getting energy from pyrophosphate

Página do item simplificado
dc.contributor.authorSuzuki, J. [UNESP]
dc.contributor.authorMutton, M. A. [UNESP]
dc.contributor.authorFerro, M. I T [UNESP]
dc.contributor.authorLemos, M. V F [UNESP]
dc.contributor.authorPizauro, J. M. [UNESP]
dc.contributor.authorMutton, M. J R [UNESP]
dc.contributor.authorDi Mauro, S. M Z [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-27T11:20:59Z
dc.date.available2014-05-27T11:20:59Z
dc.date.issued2003-12-01
dc.description.abstractPyrophosphate-dependent phosphofructokinase (PPi-PFK) has been detected in several types of plant cells, but the gene has not been reported in sugar cane. Using Citrux paradixi PPi-PFK gene (AF095520 and AF095521) sequences to search the sugar cane EST database, we have identified both the α and β subunits of this enzyme. The deduced amino acid sequences showed 76 and 80% similarity with the corresponding α and β subunits of C. paradisi. A high degree of similarity was also observed among the PFK β subunits when the alignment of the sugar cane sequences was compared to those of Ricinus communis and Solanum tuberosum, it appears that α and β are two distinct subunits; they were found at different concentrations in several sugar cane tissues. It remains to be determined if the different gene expression levels have some physiological importance and how they affect sucrose synthesis, export, and storage in vacuoles. A comparison between the amino acid sequences of β PFKs from a variety of organisms allowed us to identify the two critical Asp residues typical of this enzyme's activity site and the other binding sites; these residues are tightly conserved in all members of this protein family. Apparently, there are catalytic residues on the β subunit of the pyrophosphate-dependent enzyme.en
dc.description.affiliationDepartamento de Tecnologia Fac. Cie. Agrarias/Vet. Jaboticabal UNESP, Jaboticabal, SP
dc.description.affiliationBrazilian Clone Collection Center Fac. Cie. Agrarias/Vet. Jaboticabal UNESP, Jaboticabal, SP
dc.description.affiliationDepartamento de Producao Vegetal Fac. Cie. Agrarias/Vet. Jaboticabal UNESP, Jaboticabal, SP
dc.description.affiliationDepto. de Biol. Apl. a Agropecuaria Fac. Cie. Agrarias/Vet. Jaboticabal UNESP, Jaboticabal, SP
dc.description.affiliationUnespDepartamento de Tecnologia Fac. Cie. Agrarias/Vet. Jaboticabal UNESP, Jaboticabal, SP
dc.description.affiliationUnespBrazilian Clone Collection Center Fac. Cie. Agrarias/Vet. Jaboticabal UNESP, Jaboticabal, SP
dc.description.affiliationUnespDepartamento de Producao Vegetal Fac. Cie. Agrarias/Vet. Jaboticabal UNESP, Jaboticabal, SP
dc.description.affiliationUnespDepto. de Biol. Apl. a Agropecuaria Fac. Cie. Agrarias/Vet. Jaboticabal UNESP, Jaboticabal, SP
dc.format.extent376-382
dc.identifierhttp://www.geneticsmr.com//year2003/vol2-4/pdf/gmr0065.pdf
dc.identifier.citationGenetics and Molecular Research, v. 2, n. 4, p. 376-382, 2003.
dc.identifier.file2-s2.0-2542463107.pdf
dc.identifier.issn1676-5680
dc.identifier.lattes8951141172579985
dc.identifier.lattes7179273060624761
dc.identifier.lattes2663920223082171
dc.identifier.scopus2-s2.0-2542463107
dc.identifier.urihttp://hdl.handle.net/11449/67545
dc.language.isoeng
dc.relation.ispartofGenetics and Molecular Research
dc.relation.ispartofsjr0,439
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectATP
dc.subjectPPi-PFK
dc.subjectPyrophosphate phosphofructose 1-kinase
dc.subjectSucrose
dc.subjectSucrose synthase
dc.subjectSugar cane
dc.subjectcomplementary DNA
dc.subjectinorganic pyrophosphatase
dc.subjectphosphotransferase
dc.subjectpyrophosphate fructose 6 phosphate 1 phosphotransferase
dc.subjectpyrophosphate-fructose 6-phosphate 1-phosphotransferase
dc.subjectpyrophosphate
dc.subjectpyrophosphate phosphofructose 1 kinase
dc.subjectsucrose
dc.subjectunclassified drug
dc.subjectamino acid sequence
dc.subjectenzymology
dc.subjectgenetics
dc.subjectmetabolism
dc.subjectmolecular genetics
dc.subjectsugarcane
dc.subjectalpha chain
dc.subjectbeta chain
dc.subjectcarbohydrate synthesis
dc.subjectDNA library
dc.subjectenergy metabolism
dc.subjectenzyme active site
dc.subjectenzyme binding
dc.subjectenzyme subunit
dc.subjectexpressed sequence tag
dc.subjectgene expression
dc.subjectgene identification
dc.subjectgrapefruit
dc.subjectnonhuman
dc.subjectplant genetics
dc.subjectplant tissue
dc.subjectpotato
dc.subjectRicinus communis
dc.subjectspecies comparison
dc.subjecttissue level
dc.subjectAmino Acid Sequence
dc.subjectDNA, Complementary
dc.subjectMolecular Sequence Data
dc.subjectPhosphotransferases
dc.subjectPyrophosphatases
dc.subjectSaccharum
dc.subjectArundinaria
dc.subjectCitrus x paradisi
dc.subjectSaccharum hybrid cultivar
dc.subjectSolanum
dc.subjectSolanum tuberosum
dc.titlePutative pyrophosphate phosphofructose 1-kinase genes identified in sugarcane may be getting energy from pyrophosphateen
dc.typeArtigo
dcterms.licensehttp://www.geneticsmr.com/node/5
unesp.author.lattes8951141172579985
unesp.author.lattes7179273060624761
unesp.author.lattes2663920223082171
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Agrárias e Veterinárias, Jaboticabalpt

Arquivos

Pacote Original
Agora exibindo 1 - 1 de 1
Imagem de Miniatura
Nome:
2-s2.0-2542463107.pdf
Tamanho:
557.29 KB
Formato:
Adobe Portable Document Format
Baixar

Coleções

Artigos - Tecnologia - FCAV
Artigos - Biologia - FCAV
Artigos - Produção Vegetal - FCAV