Purification, crystallization and preliminary X-ray diffraction analysis of crotamine, a myotoxic polypeptide from the Brazilian snake Crotalus durissus terrificus

Página do item simplificado
dc.contributor.authorCoronado, Monika A. [UNESP]
dc.contributor.authorGeorgieva, Dessislava
dc.contributor.authorBuck, Friedrich
dc.contributor.authorGabdoulkhakov, Azat H.
dc.contributor.authorUllah, Anwar [UNESP]
dc.contributor.authorSpencer, Patrick J.
dc.contributor.authorArni, Raghuvir K. [UNESP]
dc.contributor.authorBetzel, Christian
dc.contributor.institutionUniv Hamburg
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniv Med Ctr Hamburg Eppendorf
dc.contributor.institutionRussian Acad Sci
dc.contributor.institutionInstituto de Pesquisas Energéticas e Nucleares (IPEN)
dc.date.accessioned2014-05-20T14:02:42Z
dc.date.available2014-05-20T14:02:42Z
dc.date.issued2012-09-01
dc.description.abstractCrotamine, a highly basic myotoxic polypeptide (molecular mass 4881 Da) isolated from the venom of the Brazilian rattlesnake Crotalus durissus terrificus, causes skeletal muscle contraction and spasms, affects the functioning of voltage-sensitive sodium channels by inducing sodium influx and possesses antitumour activity, suggesting potential pharmaceutical applications. Crotamine was purified from C. durissus terrificus venom; the crystals diffracted to 1.9 angstrom resolution and belonged to the orthorhombic space group I212121 or I222, with unit-cell parameters a = 67.75, b = 74.4, c = 81.01 angstrom. The self-rotation function indicated that the asymmetric unit contained three molecules. However, structure determination by molecular replacement using NMR-determined coordinates was unsuccessful and a search for potential derivatives has been initiated.en
dc.description.affiliationUniv Hamburg, DESY, Lab Struct Biol Infect & Inflammat, D-22603 Hamburg, Germany
dc.description.affiliationUniv Estadual Paulista UNESP, Dept Fis, Ctr Multiusuario Inovacao Biomol, BR-15054000 Sao Jose do Rio Preto, SP, Brazil
dc.description.affiliationUniv Med Ctr Hamburg Eppendorf, Inst Clin Chem, D-20246 Hamburg, Germany
dc.description.affiliationRussian Acad Sci, Inst Crystallog, Moscow 119333, Russia
dc.description.affiliationInst Pesquisas Energet & Nucl CNEN SP, Ctr Biotecnol, BR-05508000 São Paulo, Brazil
dc.description.affiliationUnespUniv Estadual Paulista UNESP, Dept Fis, Ctr Multiusuario Inovacao Biomol, BR-15054000 Sao Jose do Rio Preto, SP, Brazil
dc.description.sponsorshipDeutsche Forschungsgemeinschaft (DFG)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipDeutscher Akademischer Austauschdienst (DAAD)
dc.description.sponsorshipAlexander von Humboldt Foundation
dc.description.sponsorshipIdDFG: BE 1443-18-1
dc.description.sponsorshipIdCAPES: 80563/2911
dc.description.sponsorshipIdDAAD: PROBAL 50754442
dc.description.sponsorshipIdAlexander von Humboldt Foundation, Bonn, Germany: 3.3-BUL/1073481 STP
dc.format.extent1052-1054
dc.identifierhttp://dx.doi.org/10.1107/S1744309112032721
dc.identifier.citationActa Crystallographica Section F-structural Biology and Crystallization Communications. Hoboken: Wiley-blackwell, v. 68, p. 1052-1054, 2012.
dc.identifier.doi10.1107/S1744309112032721
dc.identifier.fileWOS000308407200011.pdf
dc.identifier.issn1744-3091
dc.identifier.lattes9162508978945887
dc.identifier.orcid0000-0003-2460-1145
dc.identifier.urihttp://hdl.handle.net/11449/22104
dc.identifier.wosWOS:000308407200011
dc.language.isoeng
dc.publisherWiley-Blackwell
dc.relation.ispartofActa Crystallographica Section F: Structural Biology and Crystallization Communications
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectcrotamineen
dc.subjectmyotoxinsen
dc.subjectCrotalus durissus terrificusen
dc.titlePurification, crystallization and preliminary X-ray diffraction analysis of crotamine, a myotoxic polypeptide from the Brazilian snake Crotalus durissus terrificusen
dc.typeArtigo
dcterms.licensehttp://journals.iucr.org/services/copyrightpolicy.html
dcterms.rightsHolderWiley-blackwell
unesp.author.lattes9162508978945887[7]
unesp.author.orcid0000-0003-2460-1145[7]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências Letras e Ciências Exatas, São José do Rio Pretopt

Arquivos

Pacote Original
Agora exibindo 1 - 1 de 1
Imagem de Miniatura
Nome:
WOS000308407200011.pdf
Tamanho:
396.6 KB
Formato:
Adobe Portable Document Format
Baixar
Licença do Pacote
Agora exibindo 1 - 2 de 2
Nenhuma Miniatura disponível
Nome:
license.txt
Tamanho:
1.71 KB
Formato:
Item-specific license agreed upon to submission
Descrição:
Nenhuma Miniatura disponível
Nome:
license.txt
Tamanho:
1.71 KB
Formato:
Item-specific license agreed upon to submission
Descrição:

Coleções

Artigos - Física - IBILCE