Novel colloidal nanocarrier of cetylpyridinium chloride: Antifungal activities on candida species and cytotoxic potential on murine fibroblasts

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dc.contributor.authorAraujo, Heitor Ceolin [UNESP]
dc.contributor.authorArias, Laís Salomão [UNESP]
dc.contributor.authorMorais Caldeirão, Anne Caroline
dc.contributor.authorDe Freitas Assumpção, Lanay Caroline
dc.contributor.authorMorceli, Marcela Grigoletto
dc.contributor.authorDe Souza Neto, Francisco Nunes [UNESP]
dc.contributor.authorDe Camargo, Emerson Rodrigues
dc.contributor.authorOliveira, Sandra Helena Penha de [UNESP]
dc.contributor.authorPessan, Juliano Pelim [UNESP]
dc.contributor.authorMonteiro, Douglas Roberto
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversity of Western São Paulo (UNOESTE)
dc.contributor.institutionUniversidade Federal de São Carlos (UFSCar)
dc.date.accessioned2021-06-25T10:36:15Z
dc.date.available2021-06-25T10:36:15Z
dc.date.issued2020-12-01
dc.description.abstractNanocarriers have been used as alternative tools to overcome the resistance of Candida species to conventional treatments. This study prepared a nanocarrier of cetylpyridinium chloride (CPC) using iron oxide nanoparticles (IONPs) conjugated with chitosan (CS), and assessed its antifungal and cytotoxic effects. CPC was immobilized on CS-coated IONPs, and the nanocarrier was physico-chemically characterized. Antifungal effects were determined on planktonic cells of Candida albicans and Candida glabrata (by minimum inhibitory concentration (MIC) assays) and on single- and dual-species biofilms of these strains (by quantification of cultivable cells, total biomass and metabolic activity). Murine fibroblasts were exposed to different concentrations of the nanocarrier, and the cytotoxic effect was evaluated by MTT reduction assay. Characterization methods confirmed the presence of a nanocarrier smaller than 313 nm. IONPs-CS-CPC and free CPC showed the same MIC values (0.78 µg mL−1). CPC-containing nanocarrier at 78 µg mL−1 significantly reduced the number of cultivable cells for all biofilms, surpassing the effect promoted by free CPC. For total biomass, metabolic activity, and cytotoxic effects, the nanocarrier and free CPC produced statistically similar outcomes. In conclusion, the IONPs-CS-CPC nanocarrier was more effective than CPC in reducing the cultivable cells of Candida biofilms without increasing the cytotoxic effects of CPC, and may be a useful tool for the treatment of oral fungal infections.en
dc.description.affiliationDepartment of Preventive and Restorative Dentistry School of Dentistry Araçatuba São Paulo State University (UNESP)
dc.description.affiliationUniversity of Western São Paulo (UNOESTE)
dc.description.affiliationSchool of Dentistry University of Western São Paulo (UNOESTE)
dc.description.affiliationDepartment of Chemistry Federal University of São Carlos (UFSCar)
dc.description.affiliationDepartment of Basic Sciences School of Dentistry São Paulo State University (UNESP)
dc.description.affiliationUnespDepartment of Preventive and Restorative Dentistry School of Dentistry Araçatuba São Paulo State University (UNESP)
dc.description.affiliationUnespDepartment of Basic Sciences School of Dentistry São Paulo State University (UNESP)
dc.format.extent1-14
dc.identifierhttp://dx.doi.org/10.3390/jof6040218
dc.identifier.citationJournal of Fungi, v. 6, n. 4, p. 1-14, 2020.
dc.identifier.doi10.3390/jof6040218
dc.identifier.issn2309-608X
dc.identifier.scopus2-s2.0-85092576738
dc.identifier.urihttp://hdl.handle.net/11449/206673
dc.language.isoeng
dc.relation.ispartofJournal of Fungi
dc.sourceScopus
dc.subjectAntifungal agents
dc.subjectBiofilms
dc.subjectCandida albicans
dc.subjectCandida glabrata
dc.subjectCetylpyridinium chloride
dc.subjectChitosan
dc.subjectIron oxide nanoparticles
dc.titleNovel colloidal nanocarrier of cetylpyridinium chloride: Antifungal activities on candida species and cytotoxic potential on murine fibroblastsen
dc.typeArtigo
unesp.author.orcid0000-0001-5229-5259[10]

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