eIF5A binds to translational machinery components and affects translation in yeast

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dc.contributor.authorZanelli, Cleslei Fernando [UNESP]
dc.contributor.authorMaragno, Ana L. C.
dc.contributor.authorGregio, Ana P. B.
dc.contributor.authorKomili, Suzanne
dc.contributor.authorPandolfi, Jose R.
dc.contributor.authorMestriner, Carlos A.
dc.contributor.authorLustri, Wilton R.
dc.contributor.authorValentini, Sandro Roberto [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionHarvard Univ
dc.date.accessioned2014-05-20T13:24:41Z
dc.date.available2014-05-20T13:24:41Z
dc.date.issued2006-10-06
dc.description.abstractThe putative translation factor eIF5A is essential for cell viability and is highly conserved from archebacteria to mammals. Although this protein was originally identified as a translation initiation factor, subsequent experiments did not support a role for eIF5A in general translation. In this work, we demonstrate that eIF-5A interacts with structural components of the 80S ribosome, as well as with the translation elongation factor 2 (eEF2). Moreover, eIF5A is further shown to cofractionate with monosomes in a translation-dependent manner. Finally, eIF5A mutants show altered polysome profiles and are sensitive to translation inhibitors. Our results re-establish a function for eIF5A in translation and suggest a role for this factor in translation elongation instead of translation initiation. (c) 2006 Elsevier B.V. All rights reserved.en
dc.description.affiliationUNESP, Sch Pharmaceut Sci, Dept Biol Sci, BR-14801902 Araraquara, SP, Brazil
dc.description.affiliationHarvard Univ, Sch Med, Dept Syst Biol, Boston, MA 02115 USA
dc.description.affiliationHarvard Univ, Sch Med, Dept Biol Chem & Mol Pharmacol, Boston, MA 02115 USA
dc.description.affiliationUnespUNESP, Sch Pharmaceut Sci, Dept Biol Sci, BR-14801902 Araraquara, SP, Brazil
dc.format.extent1358-1366
dc.identifierhttp://dx.doi.org/10.1016/j.bbrc.2006.07.195
dc.identifier.citationBiochemical and Biophysical Research Communications. San Diego: Academic Press Inc. Elsevier B.V., v. 348, n. 4, p. 1358-1366, 2006.
dc.identifier.doi10.1016/j.bbrc.2006.07.195
dc.identifier.issn0006-291X
dc.identifier.lattes5333250355049814
dc.identifier.lattes1525665408900195
dc.identifier.orcid0000-0001-7831-1149
dc.identifier.urihttp://hdl.handle.net/11449/7744
dc.identifier.wosWOS:000240425600020
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofBiochemical and Biophysical Research Communications
dc.relation.ispartofjcr2.559
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjecteIF5Apt
dc.subjectribosomept
dc.subjecttranslationpt
dc.subjecteEF2pt
dc.subjectP0pt
dc.subjectL11pt
dc.subjecthypusinept
dc.subjectprotein synthesispt
dc.titleeIF5A binds to translational machinery components and affects translation in yeasten
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.
unesp.author.lattes5333250355049814
unesp.author.lattes1525665408900195[1]
unesp.author.orcid0000-0001-7831-1149[1]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de CiĂȘncias FarmacĂȘuticas, Araraquarapt

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