Potential Use of Brazilian Green Propolis Extracts as New Photosensitizers for Antimicrobial Photodynamic Therapy against Cariogenic Microorganisms

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dc.contributor.authorde Oliveira, Analú Barros [UNESP]
dc.contributor.authorFerrisse, Túlio Morandin [UNESP]
dc.contributor.authorFrança, Gabriela Gomes [UNESP]
dc.contributor.authorde Annunzio, Sarah Raquel [UNESP]
dc.contributor.authorKopp, Willian [UNESP]
dc.contributor.authorFontana, Carla Raquel [UNESP]
dc.contributor.authorBrighenti, Fernanda Lourenção [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.date.accessioned2023-07-29T13:43:27Z
dc.date.available2023-07-29T13:43:27Z
dc.date.issued2023-02-01
dc.description.abstractThe synergic effect of Streptococcus mutans and Candida albicans increases dental caries severity. Antimicrobial photodynamic therapy (aPDT) is a non-invasive treatment for antimicrobial aspects. However, the current photosensitizers (PS) have many downsides for dental applications. This study aimed to evaluate the efficiency of two different Brazilian green propolis (BGP-AF and BGP-AG) as PS for aPDT against these microorganisms. A single-species biofilm was irradiated with crude extracts and their fractions and controls. Such extracts showed the best results and were evaluated in dual-species biofilms. Photodegradation, reactive oxygen species (ROS), cytotoxicity, and color stability assays were also investigated. Reductions higher than 3 log10 CFU/mL (p < 0.0001) occurred for crude BGP in single- and dual-species biofilms. Singlet oxygen was produced in BGP (p < 0.0001). BGP-mediated aPDT delayed S. mutans and C. albicans regrowth after 24 h of treatment (p < 0.0001). Both BGP did not change the color of dental materials (p > 0.05). BGP-AF-mediated aPDT showed 72.41% of oral keratinocyte viability (p < 0.0001). BGP extracts may be used in aPDT against S. mutans and C. albicans. Specifically, BGP-AF may represent a promising PS for dental applications.en
dc.description.affiliationDepartment of Morphology and Pediatric Dentistry School of Dentistry São Paulo State University (UNESP), SP
dc.description.affiliationDepartment of Dental Materials and Prosthodontics School of Dentistry São Paulo State University (UNESP), SP
dc.description.affiliationDepartment of Clinical Analysis School of Pharmaceutical Sciences São Paulo State University (UNESP), SP
dc.description.affiliationUnespDepartment of Morphology and Pediatric Dentistry School of Dentistry São Paulo State University (UNESP), SP
dc.description.affiliationUnespDepartment of Dental Materials and Prosthodontics School of Dentistry São Paulo State University (UNESP), SP
dc.description.affiliationUnespDepartment of Clinical Analysis School of Pharmaceutical Sciences São Paulo State University (UNESP), SP
dc.identifierhttp://dx.doi.org/10.3390/pathogens12020155
dc.identifier.citationPathogens, v. 12, n. 2, 2023.
dc.identifier.doi10.3390/pathogens12020155
dc.identifier.issn2076-0817
dc.identifier.scopus2-s2.0-85148759659
dc.identifier.urihttp://hdl.handle.net/11449/248414
dc.language.isoeng
dc.relation.ispartofPathogens
dc.sourceScopus
dc.subjectantimicrobial photodynamic therapy
dc.subjectBrazilian green propolis
dc.subjectcariogenic microorganisms
dc.subjectnatural photosensitizer
dc.titlePotential Use of Brazilian Green Propolis Extracts as New Photosensitizers for Antimicrobial Photodynamic Therapy against Cariogenic Microorganismsen
dc.typeArtigo
unesp.author.orcid0000-0002-7177-4519[1]
unesp.author.orcid0000-0002-9933-2819[2]
unesp.author.orcid0000-0002-9135-3690[6]
unesp.author.orcid0000-0002-4470-5171[7]

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