Performance of IFAT, ELISA, direct parasitological examination and PCR on lymph node aspirates for canine visceral leishmaniasis diagnosis

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dc.contributor.authorCamargo, J. B. [UNESP]
dc.contributor.authorLangoni, Hélio [UNESP]
dc.contributor.authorTroncarelli, M. Z. [UNESP]
dc.contributor.authorMachado, J. G. [UNESP]
dc.contributor.authorLucheis, Simone Baldini [UNESP]
dc.contributor.authorPadovani, C. R. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2014-05-20T13:40:00Z
dc.date.available2014-05-20T13:40:00Z
dc.date.issued2010-01-01
dc.description.abstractCanine visceral leishmaniasis (CVL) is endemic in numerous Brazilian regions. The greatest difficulty in controlling the disease is the diagnostic limitation. In the present study, the most common tests employed for visceral leishmaniasis diagnosis were compared: immunofluorescence antibody test (IFAT), immunoenzymatic assay (ELISA), direct parasitological examination and polymerase chain reaction (PCR). Samples of lymph node aspirates and blood were collected from 100 dogs that lived in an endemic area (Bauru city, São Paulo state) and from 100 negative controls from a non-endemic area (Botucatu city, São Paulo state). Specificity of both IFAT and PCR was 100% whereas ELISA was 99%. Sensitivities were 97.77, 93.33 and 91.11% respectively for IFAT, ELISA and PCR.en
dc.description.affiliationUNESP Univ Estadual Paulista, São Paulo State Univ, Sch Vet Med & Anim Husb, Dept Vet Hyg & Publ Hlth, Botucatu, SP, Brazil
dc.description.affiliationAPTA SAA, São Paulo Agcy Agribusiness Technol, Bauru, SP, Brazil
dc.description.affiliationUNESP Univ Estadual Paulista, São Paulo State Univ, Dept Biostat, Botucatu Biosci Inst, Botucatu, SP, Brazil
dc.description.affiliationUnespUNESP Univ Estadual Paulista, São Paulo State Univ, Sch Vet Med & Anim Husb, Dept Vet Hyg & Publ Hlth, Botucatu, SP, Brazil
dc.description.affiliationUnespUNESP Univ Estadual Paulista, São Paulo State Univ, Dept Biostat, Botucatu Biosci Inst, Botucatu, SP, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.format.extent414-420
dc.identifierhttp://dx.doi.org/10.1590/S1678-91992010000300007
dc.identifier.citationJournal of Venomous Animals and Toxins Including Tropical Diseases. Botucatu: Cevap-unesp, v. 16, n. 3, p. 414-420, 2010.
dc.identifier.fileS1678-91992010000300007-en.pdf
dc.identifier.issn1678-9199
dc.identifier.lattes8727897080522289
dc.identifier.scieloS1678-91992010000300007
dc.identifier.urihttp://hdl.handle.net/11449/13899
dc.identifier.wosWOS:000281692600007
dc.language.isoeng
dc.publisherUniversidade Estadual Paulista (Unesp), Centro de Estudos de Venenos e Animais Peçonhentos (CEVAP)
dc.relation.ispartofJournal of Venomous Animals and Toxins Including Tropical Diseases
dc.relation.ispartofjcr1.782
dc.relation.ispartofsjr0,573
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.subjectvisceral leishmaniasisen
dc.subjectdogen
dc.subjectdiagnosisen
dc.titlePerformance of IFAT, ELISA, direct parasitological examination and PCR on lymph node aspirates for canine visceral leishmaniasis diagnosisen
dc.typeTrabalho apresentado em evento
dcterms.licensehttp://www.scielo.br/revistas/jvatitd/iaboutj.htm
dcterms.rightsHolderCevap-unesp
unesp.author.lattes8727897080522289[6]
unesp.author.orcid0000-0002-7719-9682[6]
unesp.author.orcid0000-0001-5127-0762[2]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatupt
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina Veterinária e Zootecnia, Botucatupt

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