Melittin induces in vitro death of Leishmania (Leishmania) infantum by triggering the cellular innate immune response

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dc.contributor.authorPereira, Andreia Vieira [UNESP]
dc.contributor.authorde Barros, Gustavo [UNESP]
dc.contributor.authorPinto, Erika Gracielle
dc.contributor.authorTempone, Andre Gustavo
dc.contributor.authorOrsi, Ricardo de Oliveira [UNESP]
dc.contributor.authordos Santos, Lucilene Delazari [UNESP]
dc.contributor.authorCalvi, Sueli [UNESP]
dc.contributor.authorFerreira, Rui Seabra [UNESP]
dc.contributor.authorPimenta, Daniel Carvalho
dc.contributor.authorBarraviera, Benedito [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionAdolfo Lutz Institute
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionLaboratory of Biochemistry and Biophysics
dc.date.accessioned2018-12-11T17:00:03Z
dc.date.available2018-12-11T17:00:03Z
dc.date.issued2016-01-08
dc.description.abstractBackground: Apis mellifera venom, which has already been recommended as an alternative anti-inflammatory treatment, may be also considered an important source of candidate molecules for biotechnological and biomedical uses, such as the treatment of parasitic diseases. Methods: Africanized honeybee venom from Apis mellifera was fractionated by RP-C18-HPLC and the obtained melittin was incubated with promastigotes and intracellular amastigotes of Leishmania (L.) infantum. Cytotoxicity to mice peritoneal macrophages was evaluated through mitochondrial oxidative activity. The production of anti- and pro-inflammatory cytokines, NO and H2O2 by macrophages was determined. Results: Promastigotes and intracellular amastigotes were susceptible to melittin (IC50 28.3 μg.mL-1 and 1.4 μg.mL-1, respectively), but also showed mammalian cell cytotoxicity with an IC50 value of 5.7 μg.mL-1. Uninfected macrophages treated with melittin increased the production of IL-10, TNF-aα, NO and H2O2. Infected melittin-treated macrophages increased IL-12 production, but decreased the levels of IL-10, TNF-aα, NO and H2O2. Conclusions: The results showed that melittin acts in vitro against promastigotes and intracellular amastigotes of Leishmania (L.) infantum. Furthermore, they can act indirectly on intracellular amastigotes through a macrophage immunomodulatory effect.en
dc.description.affiliationSão Paulo State University (UNESP - Univ Estadual Paulista) Graduate Program in Tropical Diseases Botucatu Medical School
dc.description.affiliationAdolfo Lutz Institute Department of Parasitology
dc.description.affiliationUniversity of São Paulo (USP) Laboratory of Protozoology Institute for Tropical Medicine
dc.description.affiliationSão Paulo State University (UNESP - Univ Estadual Paulista) Department of Animal Production School of Veterinary Medicine and Animal Husbandry
dc.description.affiliationSão Paulo State University (UNESP - Univ Estadual Paulista) Center for the Study of Venoms and Venomous Animals (CEVAP), Rua José Barbosa de Barros, 1780
dc.description.affiliationButantan Institute Laboratory of Biochemistry and Biophysics
dc.description.affiliationUnespSão Paulo State University (UNESP - Univ Estadual Paulista) Graduate Program in Tropical Diseases Botucatu Medical School
dc.description.affiliationUnespSão Paulo State University (UNESP - Univ Estadual Paulista) Department of Animal Production School of Veterinary Medicine and Animal Husbandry
dc.description.affiliationUnespSão Paulo State University (UNESP - Univ Estadual Paulista) Center for the Study of Venoms and Venomous Animals (CEVAP), Rua José Barbosa de Barros, 1780
dc.identifierhttp://dx.doi.org/10.1186/s40409-016-0055-x
dc.identifier.citationJournal of Venomous Animals and Toxins Including Tropical Diseases, v. 22, n. 1, 2016.
dc.identifier.doi10.1186/s40409-016-0055-x
dc.identifier.fileS1678-91992016000100301.pdf
dc.identifier.issn1678-9199
dc.identifier.issn1678-9180
dc.identifier.scieloS1678-91992016000100301
dc.identifier.scopus2-s2.0-84953405951
dc.identifier.urihttp://hdl.handle.net/11449/172394
dc.language.isoeng
dc.relation.ispartofJournal of Venomous Animals and Toxins Including Tropical Diseases
dc.relation.ispartofsjr0,573
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectAntiparasitic
dc.subjectApis mellifera
dc.subjectCytokines
dc.subjectLeishmania
dc.subjectLeishmaniasis
dc.subjectMelittin
dc.subjectPeptides
dc.subjectToxins
dc.titleMelittin induces in vitro death of Leishmania (Leishmania) infantum by triggering the cellular innate immune responseen
dc.typeArtigo
unesp.author.lattes6840524602748457[10]
unesp.author.orcid0000-0002-9855-5594[10]

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Artigos - Doenças Tropicais e Diagnósticos por Imagem - FMB
Botucatu - CEVAP - Centro de Estudos de Venenos e Animais Peçonhentos