Vocal Fold Nodules: Morphological and Immunohistochemical Investigations

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dc.contributor.authorMartins, Regina Helena Garcia [UNESP]
dc.contributor.authorFaveri, Julio de [UNESP]
dc.contributor.authorDomingues, Maria Aparecida Custódio [UNESP]
dc.contributor.authorde Albuquerque e Silva, Rafael
dc.contributor.authorFabro, Alexandre [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T13:36:42Z
dc.date.available2014-05-20T13:36:42Z
dc.date.issued2010-09-01
dc.description.abstractThe objective of this study was to investigate the morphological and immunohistochemical characteristics of vocal fold nodules. The study design was prospective and retrospective. For the histological study, we reviewed 15 slides from the surgical cases of vocal fold nodules, in which we analyzed epithelium, basal membrane (bm), and lamina propria. For the transmission and scanning electron microscopy (TEM, SEM) studies, five new cases on vocal fold nodules were included. Immunohistochemistry study was carried out in the 15 specimens, using antifibronectin, antilaminin, and anticollagen IV antibodies. The main histological alterations were epithelial hyperplasia (73.33%), basement membrane thickening (86.66%), edema, and fibrosis (93.33%). SEM-reduction in mucous lacing and increase in the desquamating cells, without epithelial erosion. TEM-hyperplasia of the epithelium, enlargement of the intercellular junctions, which was filled by fluid, subepithelial thickening of the lamina reticularis, and break points in the basal membrane. Immunohistochemistry-we identified greater immunoexpression of fibronectin on the basal membrane, on the lamina propria, and around the vessels. Antilaminin and anticollagen IV antibodies showed higher pigmentation on the endothelium of the vessels than that on the basal membrane. In vocal fold nodules, combined assessment using light microscopy, electron microscopy, and immunohistochemistry can reveal important morphological details useful in characterizing these lesions.en
dc.description.affiliationSão Paulo State Univ, Dept Otorhinolaryngol Ophthalmol & Head & Neck Su, Sch Med, Fac Med, São Paulo, Brazil
dc.description.affiliationSão Paulo State Univ, Dept Pathol, Sch Med, São Paulo, Brazil
dc.description.affiliationUnespSão Paulo State Univ, Dept Otorhinolaryngol Ophthalmol & Head & Neck Su, Sch Med, Fac Med, São Paulo, Brazil
dc.description.affiliationUnespSão Paulo State Univ, Dept Pathol, Sch Med, São Paulo, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.format.extent531-539
dc.identifierhttp://dx.doi.org/10.1016/j.jvoice.2009.01.002
dc.identifier.citationJournal of Voice. New York: Mosby-elsevier, v. 24, n. 5, p. 531-539, 2010.
dc.identifier.doi10.1016/j.jvoice.2009.01.002
dc.identifier.issn0892-1997
dc.identifier.lattes8683290712226765
dc.identifier.lattes0585723113037140
dc.identifier.urihttp://hdl.handle.net/11449/12635
dc.identifier.wosWOS:000281710100003
dc.language.isoeng
dc.publisherMosby-elsevier
dc.relation.ispartofJournal of Voice
dc.relation.ispartofsjr0,735
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectVocal fold nodulesen
dc.subjectMorphologyen
dc.subjectImmunohistochemistryen
dc.titleVocal Fold Nodules: Morphological and Immunohistochemical Investigationsen
dc.typeResumo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderMosby-elsevier
unesp.author.lattes8683290712226765
unesp.author.lattes0585723113037140
unesp.author.orcid0000-0002-7687-3161[5]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina, Botucatupt

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