Purification and partial characterization of cathepsin D from porcine (Sus scrofa) liver using affinity chromatography

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dc.contributor.authorCanduri, F.
dc.contributor.authorWard, R. J.
dc.contributor.authorde Azevedo, W. F.
dc.contributor.authorGomes, RAS
dc.contributor.authorArni, R. K.
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionFac Med Triangulo Mineiro
dc.date.accessioned2014-05-20T15:28:21Z
dc.date.available2014-05-20T15:28:21Z
dc.date.issued1998-07-01
dc.description.abstractCathepsin D, a lysosomal aspartic protease, has been purified from porcine liver using a combination of pepstatin-A agarose and Affi-Gel Blue affinity chromatography, followed by size-exclusion chromatography. The purified protein consists of two polypeptide chains of 15 and 30 kDa, and has an isoelectric point of 6.8. Porcine liver cathepsin D has maximum activity at pH 2.5-3.0 as determined by its activity against hemoglobin, with a K-cat of 14.3 s(-1) and a k(cat)/K-M of 2.70 x 10(6) s(-1) M-1 as determined by the hydrolysis of a fluorogenic peptide substrate.en
dc.description.affiliationUNESP, IBILCE, Dept Fis, BR-15054000 Sao Jose do Rio Preto, SP, Brazil
dc.description.affiliationFac Med Triangulo Mineiro, Dept Bioquim Celular & Biofis, BR-38015050 Uberaba, MG, Brazil
dc.description.affiliationUnespUNESP, IBILCE, Dept Fis, BR-15054000 Sao Jose do Rio Preto, SP, Brazil
dc.format.extent797-803
dc.identifierhttp://dx.doi.org/10.1080/15216549800203222
dc.identifier.citationBiochemistry and Molecular Biology International. Marrickville: Academic Press Aust, v. 45, n. 4, p. 797-803, 1998.
dc.identifier.doi10.1080/15216549800203222
dc.identifier.fileWOS000075300600019.pdf
dc.identifier.issn1039-9712
dc.identifier.lattes9162508978945887
dc.identifier.orcid0000-0003-2460-1145
dc.identifier.urihttp://hdl.handle.net/11449/38170
dc.identifier.wosWOS:000075300600019
dc.language.isoeng
dc.publisherAcademic Press Aust
dc.relation.ispartofBiochemistry and Molecular Biology International
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.subjectcathepsin Dpt
dc.subjectaspartic proteasept
dc.subjectlysosomal enzymept
dc.subjectaffinity chromatographypt
dc.subjectpepstatin Apt
dc.titlePurification and partial characterization of cathepsin D from porcine (Sus scrofa) liver using affinity chromatographyen
dc.typeArtigo
dcterms.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dcterms.rightsHolderAcademic Press Aust
unesp.author.lattes9162508978945887[5]
unesp.author.orcid0000-0003-1136-5737[2]
unesp.author.orcid0000-0002-5984-0712[4]
unesp.author.orcid0000-0003-2460-1145[5]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências Letras e Ciências Exatas, São José do Rio Pretopt

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Artigos - Física - IBILCE