X chromosome heterochromatic pattern and nucleolar synthesis in pupal ovaries of Aedes aegypti

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dc.contributor.authorde Sousa, R. D.
dc.contributor.authorBicudo, HEMD
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T15:23:27Z
dc.date.available2014-05-20T15:23:27Z
dc.date.issued1998-01-01
dc.description.abstractC-banding and silver-staining techniques were used to examine pupal ovaries of Aedes aegypti from Sao Jose do Rio Preto (Brazil). Silver staining in ovary cystocytes showed two basic patterns relative to the nucleolar morphology: viz (1) a single, compact small body; and (2) multiple bodies encompassing large nuclear areas. These two types of cystocytes were present in the ratio of 7:1, which is the same as the number of nurse cells and oocytes, respectively, in each follicle. This suggests the possibility of eventually using such a nucleolar morphological difference to recognize both cell types in developmental stages before emergence. Silver nitrate staining in metaphase chromosomes revealed centromeric bands on all six chromosomes. The C-banding pattern in metaphase chromosomes showed an intercalary band in one of the X arms, as described previously in other populations. In ovary cystocytes (pachytene stage) this C-positive band seemed to consist of two chromomeres. Phase contrast microscopy showed that the nucleolus was associated with the distal chromomere of this intercalary C-band, indicating that the nucleolus organizer region was located in that part of the heterochromatic band.en
dc.description.affiliationUNESP, Dept Biol, Inst Biociencias, Letras Ciências Exatas, BR-15054000 Sao Jose Rio Preto, SP, Brazil
dc.description.affiliationUnespUNESP, Dept Biol, Inst Biociencias, Letras Ciências Exatas, BR-15054000 Sao Jose Rio Preto, SP, Brazil
dc.format.extent185-193
dc.identifierhttp://cat.inist.fr/?aModele=afficheN&cpsidt=10566824
dc.identifier.citationCytobios. Cambridge: Faculty Press, v. 93, n. 374, p. 185-193, 1998.
dc.identifier.issn0011-4529
dc.identifier.urihttp://hdl.handle.net/11449/34238
dc.identifier.wosWOS:000076048100006
dc.language.isoeng
dc.publisherFaculty Press
dc.relation.ispartofCytobios
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectAedes aegyptipt
dc.subjectnucleoluspt
dc.subjectnucleolar organizer regionspt
dc.subjectheterochromatic patternpt
dc.titleX chromosome heterochromatic pattern and nucleolar synthesis in pupal ovaries of Aedes aegyptien
dc.typeArtigo
dcterms.rightsHolderFaculty Press
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências Letras e Ciências Exatas, São José do Rio Pretopt

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