Distribution of exoantigens and a 43-kDa glycoprotein (gp43) in the yeast and mycelial forms of Paracoccidioides brasiliensis

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dc.contributor.authorSandoval, M. P.
dc.contributor.authorDel Negro, G. M. B.
dc.contributor.authorMendes-Giannini, Maria José Soares [UNESP]
dc.contributor.authorBrito, T. de
dc.contributor.institutionInstitute of Tropical Medicine
dc.contributor.institutionMedical School
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2014-05-27T11:18:04Z
dc.date.available2014-05-27T11:18:04Z
dc.date.issued1996-03-01
dc.description.abstractObjective. Paracoccidioides brasiliensis antigens (strain 113) were located at ultrastructural level in both yeast and mycelial forms of the fungus. The reactivity of the sera employed was analysed. Materials and methods. Immunofluorescence and ultrastructural protein A-gold immunolabelling techniques were performed using two polyclonal antisera: one against P. brasiliensis exoantigens and the other against a 43-kDa glycoprotein (gp43). Immunoblotting assays were employed to define reactivity of these antisera with somatic and metabolic antigens of both forms of the fungus. Results. The techniques employed revealed in both yeast and mycelial forms of P. brasiliensis a similar antigenic distribution. The antigens deposits were seen within the cytoplasm, and over the cell wall of the fungus. The anti-exoantigen serum recognized several bands in both forms of the fungus. The anti-gp43 serum reacted strongly with the 43-kDa fraction and weakly with few other fractions. Conclusions. Immunocytochemical techniques suggest a protein synthesis within the cytoplasm followed by excretion through the cell wall. Similar results employing both polyclonal antisera were obtained.en
dc.description.affiliationDepartment of Pathology Institute of Tropical Medicine
dc.description.affiliationLaboratory of Medical Mycology University of S. Paulo Medical School
dc.description.affiliationDepartment of Clinical Analysis Faculty of Pharmaceutical Sciences UNESP, S. Paulo
dc.description.affiliationFaculdade de Medicina da USP Departamento de Patologia, Av. Dr Arnaldo, 455, 01246 903, S. Paulo, S.P.
dc.description.affiliationUnespDepartment of Clinical Analysis Faculty of Pharmaceutical Sciences UNESP, S. Paulo
dc.format.extent1-6
dc.identifier.citationJournal de Mycologie Medicale, v. 6, n. 1, p. 1-6, 1996.
dc.identifier.issn1156-5233
dc.identifier.orcid0000-0002-8059-0826
dc.identifier.scopus2-s2.0-0029797985
dc.identifier.urihttp://hdl.handle.net/11449/64759
dc.language.isoeng
dc.relation.ispartofJournal de Mycologie Medicale
dc.relation.ispartofjcr1.606
dc.relation.ispartofsjr0,495
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subjectantigens
dc.subjectParacoccidioides brasiliensis
dc.subjectultrastructural labeling
dc.subjectglycoprotein
dc.subjectpolyclonal antibody
dc.subjectantigen expression
dc.subjectcell wall
dc.subjectcontrolled study
dc.subjectimmunocytochemistry
dc.subjectimmunofluorescence
dc.subjectnonhuman
dc.subjectparacoccidioides brasiliensis
dc.subjectprotein synthesis
dc.subjectultrastructure
dc.subjectyeast
dc.titleDistribution of exoantigens and a 43-kDa glycoprotein (gp43) in the yeast and mycelial forms of Paracoccidioides brasiliensisen
dc.typeArtigo
unesp.author.orcid0000-0002-8059-0826[3]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Farmacêuticas, Araraquarapt

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