Increased DSG2 plasmatic levels identified by transcriptomic-based secretome analysis is a potential prognostic biomarker in laryngeal carcinoma

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dc.contributor.authorCury, Sarah Santiloni [UNESP]
dc.contributor.authorLapa, Rainer Marco Lopez [UNESP]
dc.contributor.authorde Mello, Julia Bette Homem
dc.contributor.authorMarchi, Fábio Albuquerque
dc.contributor.authorDomingues, Maria Aparecida Custódio [UNESP]
dc.contributor.authorPinto, Clóvis Antonio Lopes
dc.contributor.authorCarvalho, Robson Francisco [UNESP]
dc.contributor.authorde Carvalho, Genival Barbosa
dc.contributor.authorKowalski, Luiz Paulo
dc.contributor.authorRogatto, Silvia Regina
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionA.C. Camargo Cancer Center
dc.contributor.institutionCIPE – A.C. Camargo Cancer Center
dc.contributor.institutionUniversity of Southern Denmark
dc.date.accessioned2020-12-12T02:35:43Z
dc.date.available2020-12-12T02:35:43Z
dc.date.issued2020-04-01
dc.description.abstractObjectives: The tumor secretome deconvolution is a promising strategy to identify diagnostic and prognostic biomarkers. Here, transcriptomic-based secretome analysis was performed aiming to discover laryngeal squamous cell carcinomas (LSCC) biomarkers from potentially secreted proteins (PSPs). Material and Methods: The tumor expression profile (35 LSCC biopsies compared with surrounding normal tissues - SN) revealed 589 overexpressed genes. This gene list was used for secretome analysis based on laryngeal tumors and related secretome databases. Results: Forty-nine (Laryngeal tumor secretome database) and 50 (Human Protein Atlas and Cancer Secretome Database) PSPs presented an association with worse overall survival. Specifically, DSG2 overexpression was strongly correlated with poor survival and distant metastasis. DSG2 increased expression was confirmed in the LSCC dataset (LSCC = 111; SN = 12) from TCGA. A significant association between shorter survival and DSG2 overexpression was also detected. In an independent cohort of cases, we analyzed and confirmed high protein levels of DSG2 in plasma from LSCC patients. Conclusion: A set of PSPs including the circulating DSG2, were associated with shorter overall survival in LSCC. DSG2 overexpression was also correlated with distant metastasis. The high plasmatic protein levels of DSG2 suggest its potential to be tested in liquid biopsies and applied as prognostic biomarker of LSCC.en
dc.description.affiliationDepartment of Structural and Functional Biology Institute of Biosciences São Paulo State University (UNESP)
dc.description.affiliationDepartment of Chemical and Biological Sciences Institute of Biosciences São Paulo State University (UNESP)
dc.description.affiliationDepartment of Head and Neck Surgery and Otorhinolaryngology A.C. Camargo Cancer Center
dc.description.affiliationInternational Research Center CIPE – A.C. Camargo Cancer Center
dc.description.affiliationDepartment of Pathology Faculty of Medicine São Paulo State University (UNESP)
dc.description.affiliationDepartment of Pathology A.C. Camargo Cancer Center
dc.description.affiliationDepartment of Clinical Genetics University Hospital Institute of Regional Health Research University of Southern Denmark
dc.description.affiliationUnespDepartment of Structural and Functional Biology Institute of Biosciences São Paulo State University (UNESP)
dc.description.affiliationUnespDepartment of Chemical and Biological Sciences Institute of Biosciences São Paulo State University (UNESP)
dc.description.affiliationUnespDepartment of Pathology Faculty of Medicine São Paulo State University (UNESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipUddannelses- og Forskningsministeriet
dc.identifierhttp://dx.doi.org/10.1016/j.oraloncology.2020.104592
dc.identifier.citationOral Oncology, v. 103.
dc.identifier.doi10.1016/j.oraloncology.2020.104592
dc.identifier.issn1879-0593
dc.identifier.issn1368-8375
dc.identifier.scopus2-s2.0-85079538957
dc.identifier.urihttp://hdl.handle.net/11449/201558
dc.language.isoeng
dc.relation.ispartofOral Oncology
dc.sourceScopus
dc.subjectBiomarker
dc.subjectLaryngeal squamous cell carcinoma
dc.subjectLiquid biopsy
dc.subjectSecretome
dc.subjectTranscriptome
dc.titleIncreased DSG2 plasmatic levels identified by transcriptomic-based secretome analysis is a potential prognostic biomarker in laryngeal carcinomaen
dc.typeArtigo

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Artigos - Morfologia - IBB