Paper platform for determination of bumetanide in human urine samples to detect doping in sports using digital image analysis

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dc.contributor.authorLuiz, Vitor Hugo Marques [UNESP]
dc.contributor.authorLima, Liliane Spazzapam [UNESP]
dc.contributor.authorRossini, Eduardo Luiz [UNESP]
dc.contributor.authorPezza, Leonardo [UNESP]
dc.contributor.authorPezza, Helena Redigolo [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2019-10-06T17:04:31Z
dc.date.available2019-10-06T17:04:31Z
dc.date.issued2019-06-01
dc.description.abstractDevelopment and application of a new, simple, inexpensive, and eco-friendly method is presented for the quantification of bumetanide in human urine samples for the detection of doping in sports. The method involves the use of a paper platform and digital image detection and is based on the reaction between bumetanide, p-dimethylaminocinnamaldehyde (p-DAC), and HCl in a methanolic medium, yielding a colored compound in a delimited area on a qualitative filter paper. The concentrations of p-DAC and HCl were optimized by a chemometric experimental design. After the addition of the reagents to the paper, digital images were obtained by scanning in a multifunctional printer and analyzed using the RGB pattern. The linear bumetanide concentration range was 6.90 × 10 −5 –1.37 × 10 −3 mol L −1 with R 2 = 0.993 and the limits of detection (LOD) and quantification (LOQ) were 3.60 × 10 −4 and 1.09 × 10 −4 mol L −1 , respectively. A SPE clean-up step for the samples were required to eliminate the interference of urinary urea. The recoveries obtained varied from 94% to 105%, indicating the absence of significant matrix effects or interferences in urine samples after clean-up step. The proposed method was successfully applied to the analysis of bumetanide in human urine samples.en
dc.description.affiliationInstituto de Química Universidade Estadual Paulista “Júlio de Mesquita Filho” UNESP, R. Prof. Francisco Degni 55, P.O. Box 355
dc.description.affiliationUnespInstituto de Química Universidade Estadual Paulista “Júlio de Mesquita Filho” UNESP, R. Prof. Francisco Degni 55, P.O. Box 355
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdCAPES: 001
dc.description.sponsorshipIdFAPESP: 2015/21733-1
dc.format.extent43-48
dc.identifierhttp://dx.doi.org/10.1016/j.microc.2019.03.006
dc.identifier.citationMicrochemical Journal, v. 147, p. 43-48.
dc.identifier.doi10.1016/j.microc.2019.03.006
dc.identifier.issn0026-265X
dc.identifier.lattes5978908591853524
dc.identifier.scopus2-s2.0-85062465587
dc.identifier.urihttp://hdl.handle.net/11449/190166
dc.language.isoeng
dc.relation.ispartofMicrochemical Journal
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectBumetanide
dc.subjectDigital image analysis
dc.subjectDoping
dc.subjectPaper platform
dc.subjectUrine
dc.titlePaper platform for determination of bumetanide in human urine samples to detect doping in sports using digital image analysisen
dc.typeArtigo
unesp.author.lattes5978908591853524

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Artigos - Química Orgânica - IQ
Artigos - Química Analítica - IQ