(1→6)- and (1→3)(1→6)-β-glucans from Lasiodiplodia theobromae MMBJ: structural characterization and pro-inflammatory activity

dc.contributor.authorOliveira, Kassandra S. M. [UNESP]
dc.contributor.authorDi Bastiani, Mirela [UNESP]
dc.contributor.authorCordeiro, Lucimara M. C.
dc.contributor.authorCosta, Mírian F. [UNESP]
dc.contributor.authorToledo, Karina A. [UNESP]
dc.contributor.authorIacomini, Marcello
dc.contributor.authorBabosa, Aneli M.
dc.contributor.authorDekker, Robert F. H.
dc.contributor.authorNascimento, Valéria M. G. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Federal do Paraná (UFPR)
dc.contributor.institutionUniversidade Estadual de Londrina (UEL)
dc.contributor.institutionBiorefining and Biotechnology Consultancy
dc.date.accessioned2015-12-07T15:40:39Z
dc.date.available2015-12-07T15:40:39Z
dc.date.issued2015-11-20
dc.description.abstractThe chemical composition and structural characterization of exopolysaccharides from the fungus Lasiodiplodia theobromae MMBJ are described, and the immunomodulatory activity of a purified β-glucan was evaluated. L. theobromae MMBJ produced three different β-glucans. One, fraction PEPS, was a branched (1→3)(1→6)-β-glucan and was insoluble in cold water. The other two, fractions SEPS-005R and SEPS-10E, were characterized as linear (1→6)-β-glucans with molar mass of 1.8×10(6)Da and 7.0×10(3)Da, respectively. From a total of 2.2g/L of EPS produced by L. theobromae through submerged fermentation, 1.5g/L (67%) was of the branched (1→3)(1→6)-β-glucan, while 25% (w/w) were linear (1→6)-β-glucans. Tests conducted with macrophages showed that the high molar mass (1→6)-β-glucan fraction (SEPS-005R) induced a pro-inflammatory response pattern.en
dc.description.affiliationDepartamento de Ciências Biológicas, Faculdade de Ciências e Letras (FCL), Universidade Estadual Paulista (UNESP), Assis, SP, Brasil
dc.description.affiliationDepartamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná (UFPR), Curitiba, PR, Brasil
dc.description.affiliationDepartamento de Química, Universidade Estadual de Londrina (UEL), Londrina, PR, Brasil
dc.description.affiliationBiorefining and Biotechnology Consultancy, Londrina, PR, Brasil
dc.description.affiliationUnespDepartamento de Ciências Biológicas, Faculdade de Ciências e Letras (FCL), Universidade Estadual Paulista (UNESP), Assis, SP, Brasil
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipIdCNPq: 475747/2010-0
dc.format.extent539-546
dc.identifierhttp://dx.doi.org/10.1016/j.carbpol.2015.07.060
dc.identifier.citationCarbohydrate Polymers, v. 133, p. 539-546, 2015.
dc.identifier.doi10.1016/j.carbpol.2015.07.060
dc.identifier.issn1879-1344
dc.identifier.lattes5772565774304020
dc.identifier.pubmed26344312
dc.identifier.urihttp://hdl.handle.net/11449/131690
dc.language.isoeng
dc.publisherElsevier B. V.
dc.relation.ispartofCarbohydrate Polymers
dc.rights.accessRightsAcesso restrito
dc.sourcePubMed
dc.subjectLasiodiplodanen
dc.subjectLinear (1→6)--glucanen
dc.subject(1→3)(1→6)--Glucanen
dc.subjectExopolysaccharidesen
dc.subjectImmunomodulatory activityen
dc.title(1→6)- and (1→3)(1→6)-β-glucans from Lasiodiplodia theobromae MMBJ: structural characterization and pro-inflammatory activityen
dc.typeArtigo
dcterms.rightsHolderElsevier B. V.
unesp.author.lattes5772565774304020
unesp.author.orcid0000-0002-5311-5667[1]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências e Letras, Assispt
unesp.departmentCiências Biológicas - FCLASpt

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