Microdissection testicular sperm extraction causes spermatogenic alterations in the contralateral testis

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dc.contributor.authorOliveira Filho, A. B.
dc.contributor.authorSouza, R. S. [UNESP]
dc.contributor.authorAzeredo-Oliveira, M. T. V. [UNESP]
dc.contributor.authorPeruquetti, R. L. [UNESP]
dc.contributor.authorCedenho, A. P.
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2015-03-18T15:56:27Z
dc.date.available2015-03-18T15:56:27Z
dc.date.issued2010-01-01
dc.description.abstractTesticular sperm extraction (TESE) associated with intracytoplasmic sperm injection has allowed many men presenting non-obstructive azoospermia to achieve fatherhood. Microdissection TESE (microTESE) was proposed as a method to improve sperm retrieval rates in these patients; however, there have been failures. Little is known about whether microTESE leads to spermatogenic alterations in the contralateral testis. We assessed histological outcomes of experimental microTESE in the contralateral testis of adult male rabbits. Nine adult male rabbits were divided into three groups: control (testicular biopsy to observe normal histological and morphometric values), sham (incision of the tunica vaginalis, and a contralateral testicular biopsy to observe histological and morphometric patterns, 45 days later), and study (left testicular microTESE, and a right testicular biopsy to observe histological and morphometric patterns, 45 days later). Sections were assessed by calculating Johnsen-like scores, and measuring total tubule diameter, lumen diameter and epithelial height. The results were compared using ANOVA and Bonferroni's statistical analysis. Morphometric evaluation of the seminiferous tubules did not demonstrate differences between the three groups. However, microTESE caused spermatogenic alterations, leading to maturation arrest in the contralateral testis.en
dc.description.affiliationUniv Fed Sao Paulo, Dept Cirurgia, Div Urol, Secao Reprod Humana, Sao Paulo, Brazil
dc.description.affiliationUniv Estadual Paulista, Dept Biol, Inst Biociencias Letras & Ciencias Exatas Sao Jos, Sao Jose Do Rio Preto, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Dept Biol, Inst Biociencias Letras & Ciencias Exatas Sao Jos, Sao Jose Do Rio Preto, SP, Brazil
dc.format.extent1405-1413
dc.identifierhttp://dx.doi.org/10.4238/vol9-3gmr860
dc.identifier.citationGenetics And Molecular Research. Ribeirao Preto: Funpec-editora, v. 9, n. 3, p. 1405-1413, 2010.
dc.identifier.doi10.4238/vol9-3gmr860
dc.identifier.fileWOS000280397000014.pdf
dc.identifier.issn1676-5680
dc.identifier.urihttp://hdl.handle.net/11449/117570
dc.identifier.wosWOS:000280397000014
dc.language.isoeng
dc.publisherFunpec-editora
dc.relation.ispartofGenetics And Molecular Research
dc.relation.ispartofsjr0,439
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.subjectTestis biopsyen
dc.subjectMicrodissectionen
dc.subjectSeminiferous tubulesen
dc.subjectSurgeryen
dc.titleMicrodissection testicular sperm extraction causes spermatogenic alterations in the contralateral testisen
dc.typeArtigo
dcterms.rightsHolderFunpec-editora
unesp.author.orcid0000-0003-3153-6599[4]
unesp.author.orcid0000-0003-2111-638X[3]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências Letras e Ciências Exatas, São José do Rio Pretopt

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