Treatment with cyclic adenosine monophosphate modulators prior to in vitro maturation alters the lipid composition and transcript profile of bovine cumulus-oocyte complexes and blastocysts

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dc.contributor.authorRazza, Eduardo M.
dc.contributor.authorSudano, Mateus J.
dc.contributor.authorFontes, Patricia K.
dc.contributor.authorFranchi, Fernanda F.
dc.contributor.authorBelaz, Katia Roberta A.
dc.contributor.authorSantos, Priscila H.
dc.contributor.authorCastilho, Anthony C. S.
dc.contributor.authorRocha, Daniele F. O.
dc.contributor.authorEberlin, Marcos N.
dc.contributor.authorMachado, Mariana F.
dc.contributor.authorNogueira, Marcelo F. G.
dc.contributor.institutionNove de Julho University
dc.contributor.institutionFederal University of Pampa
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.contributor.institutionKm 572 - Bairro Limoeiro
dc.date.accessioned2022-04-29T08:45:21Z
dc.date.available2022-04-29T08:45:21Z
dc.date.issued2018-01-01
dc.description.abstractMammalian oocytes resume meiosis spontaneously after removal from the ovarian follicle. We tested the effects of a 2-h prematuration treatment (Pre-IVM) with forskolin (FSK) and 3-isobutyl-1-methylxanthine (IBMX) in bovine cumulus-oocyte complexes (COCs) on the lipid content of oocytes and blastocysts, on the membrane lipid composition of blastocysts and on the transcriptional profiling of cumulus cells and blastocysts in a high-throughput platform. Embryonic development rates to the morula (mean 56.1%) or blastocyst (mean 26.3%) stages were unaffected by treatment. Lipid content was not affected after Pre-IVM, but was increased after IVM in treated oocytes. Conversely, the lipid content was reduced in Pre-IVM blastocysts. Pre-IVM COCs generated blastocysts containing blastomeres with more unsaturated lipids in their membranes. Pre-IVM also altered the relative abundance of 31 gene transcripts after 2 h and 16 transcripts after 24 h in cumulus cells, while seven transcripts were altered in blastocysts. Our results suggest that the Pre-IVM treatment affected the lipid composition and transcriptional profiles of COCs and blastocysts. Therefore, Pre-IVM with FSK and IBMX could be used either to prevent spontaneous meiotic resumption during IVM or to modulate lipid composition in the membrane and cytoplasm of blastocysts, potentially improving bovine embryos.en
dc.description.affiliationNove de Julho University, Rua Nicolau Assis, 15
dc.description.affiliationSchool of Veterinary Medicine Federal University of Pampa, Caixa Postal 118
dc.description.affiliationDepartment of Pharmacology Institute of Bioscience University of São Paulo State, Distrito de Rubião Junior s/n
dc.description.affiliationThoMSon Mass Spectrometry Laboratory Institute of Chemistry University of Campinas-UNICAMP, Rua Sérgio Buarque de Holanda s/n
dc.description.affiliationUniversity of Western São Paulo Rodovia Raposo Tavares Km 572 - Bairro Limoeiro
dc.description.affiliationDepartment of Biological Sciences Faculty of Sciences and Letters University of São Paulo State, Avenuenida Dom Antonio, 2100
dc.format.extent1314-1328
dc.identifierhttp://dx.doi.org/10.1071/RD17335
dc.identifier.citationReproduction, Fertility and Development, v. 30, n. 10, p. 1314-1328, 2018.
dc.identifier.doi10.1071/RD17335
dc.identifier.issn1448-5990
dc.identifier.issn1031-3613
dc.identifier.scopus2-s2.0-85049186989
dc.identifier.urihttp://hdl.handle.net/11449/231418
dc.language.isoeng
dc.relation.ispartofReproduction, Fertility and Development
dc.sourceScopus
dc.subjectcAMP
dc.subjectgene expression
dc.subjectmass spectrometry
dc.titleTreatment with cyclic adenosine monophosphate modulators prior to in vitro maturation alters the lipid composition and transcript profile of bovine cumulus-oocyte complexes and blastocystsen
dc.typeArtigo

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