Simulated physiological oocyte maturation has side effects on bovine oocytes and embryos

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dc.contributor.authorRazza, Eduardo M. [UNESP]
dc.contributor.authorPedersen, Hanne S.
dc.contributor.authorStroebech, Lotte
dc.contributor.authorFontes, Patricia K. [UNESP]
dc.contributor.authorKadarmideen, Haja N.
dc.contributor.authorCallesen, Henrik
dc.contributor.authorPihl, Maria
dc.contributor.authorNogueira, Marcelo F. G. [UNESP]
dc.contributor.authorHyttel, Poul
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionAarhus University
dc.contributor.institutionEmbryoTrans Biotech
dc.contributor.institutionTechnical University of Denmark
dc.contributor.institutionUniversity of Copenhagen
dc.date.accessioned2019-10-06T16:05:55Z
dc.date.available2019-10-06T16:05:55Z
dc.date.issued2019-03-15
dc.description.abstractPurpose: Oocyte maturation is a complex process involving nuclear and cytoplasmic modulations, during which oocytes acquire their ability to become fertilized and support embryonic development. The oocyte is apparently “primed” for maturation during its development in the dominant follicle. As bovine oocytes immediately resume meiosis when cultured, it was hypothesized that delaying resumption of meiosis with cyclic nucleotide modulators before in vitro maturation (IVM) would allow the oocytes to acquire improved developmental competence. Methods: We tested the Simulated Physiological Oocyte Maturation (SPOM) system that uses forskolin and 3-isobutyl-1-methylxanthine for 2 h prior to IVM against two different systems of conventional IVM (Con-IVM). We evaluated the ultrastructure of matured oocytes and blastocysts and also assessed the expression of 96 genes related to embryo quality in the blastocysts. Results: In summary, the SPOM system resulted in lower blastocyst rates than both Con-IVM systems (30 ± 9.1 vs. 35 ± 8.7; 29 ± 2.6 vs. 38 ± 2.8). Mature SPOM oocytes had significantly increased volume and number of vesicles, reduced volume and surface density of large smooth endoplasmic reticulum clusters, and lower number of mitochondria than Con-IVM oocytes. SPOM blastocysts showed only subtle differences with parallel undulations of adjacent trophectoderm plasma membranes and peripherally localized ribosomes in cells of the inner cell mass compared with Con-IVM blastocysts. SPOM blastocysts, however, displayed significant downregulation of genes related to embryonic developmental potential when compared to Con-IVM blastocysts. Conclusions: Our results show that the use of the current version of the SPOM system may have adverse effects on oocytes and blastocysts calling for optimized protocols for improving oocyte competence.en
dc.description.affiliationDepartment of Pharmacology Institute of Bioscience São Paulo State University (UNESP), Distrito de Rubião Junior s/n
dc.description.affiliationDepartment of Animal Science Aarhus University
dc.description.affiliationEmbryoTrans Biotech, Frederiksberg C
dc.description.affiliationDepartment of Bio and Health Informatics Technical University of Denmark, Kemitorvet
dc.description.affiliationDepartment of Veterinary and Animal Sciences University of Copenhagen
dc.description.affiliationDepartment of Biological Sciences School of Sciences and Languages São Paulo State University (UNESP), Avenida Dom Antonio, 2100
dc.description.affiliationUnespDepartment of Pharmacology Institute of Bioscience São Paulo State University (UNESP), Distrito de Rubião Junior s/n
dc.description.affiliationUnespDepartment of Biological Sciences School of Sciences and Languages São Paulo State University (UNESP), Avenida Dom Antonio, 2100
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipInnovationsfonden
dc.description.sponsorshipIdFAPESP: #12234099
dc.description.sponsorshipIdFAPESP: #12505332
dc.description.sponsorshipIdFAPESP: #13050831
dc.format.extent413-424
dc.identifierhttp://dx.doi.org/10.1007/s10815-018-1365-4
dc.identifier.citationJournal of Assisted Reproduction and Genetics, v. 36, n. 3, p. 413-424, 2019.
dc.identifier.doi10.1007/s10815-018-1365-4
dc.identifier.issn1573-7330
dc.identifier.issn1058-0468
dc.identifier.scopus2-s2.0-85056720403
dc.identifier.urihttp://hdl.handle.net/11449/188371
dc.language.isoeng
dc.relation.ispartofJournal of Assisted Reproduction and Genetics
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectBovine blastocyst
dc.subjectGene expression
dc.subjectIn vitro maturation
dc.subjectSPOM
dc.subjectUltrastructure
dc.titleSimulated physiological oocyte maturation has side effects on bovine oocytes and embryosen
dc.typeArtigo
unesp.author.orcid0000-0002-5193-0596[1]

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