Differential behavior of non-albicans Candida species in the central nervous system of immunocompetent and immunosuppressed mice

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dc.contributor.authorSanches, Marcelo D'Alessandre [UNESP]
dc.contributor.authorMimura, Luiza A.N. [UNESP]
dc.contributor.authorOliveira, Larissa R.C. [UNESP]
dc.contributor.authorIshikawa, Larissa L.W. [UNESP]
dc.contributor.authorGarces, Hans G. [UNESP]
dc.contributor.authorBagagli, Eduardo [UNESP]
dc.contributor.authorSartori, Alexandrina [UNESP]
dc.contributor.authorKurokawa, Cilmery Suemi [UNESP]
dc.contributor.authorFraga-Silva, Thais F.C. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2019-10-06T17:07:34Z
dc.date.available2019-10-06T17:07:34Z
dc.date.issued2019-01-01
dc.description.abstractThe genus Candida includes commensal fungi that can cause local and systemic infections, frequently involving vital organs as the central nervous system (CNS). Candida spp. occupy the fourth place among infections that affect the CNS. Although the incidence of Candida albicans is decreasing among patients under immunosuppressive therapies, the incidence of non-albicans Candida is increasing. In this context, the objective of this work was to evaluate the ability of non-albicans Candida species to spread to the CNS of immunocompetent and immunosuppressed mice. Adult female C57BL/6 mice were treated with prednisolone, intravenously infected with Candida glabrata, Candida krusei and Candida parapsilosis yeasts and then evaluated at the 3rd and 14th days after infection. All Candida species disseminated to the brain from immunocompetent animals and induced local inflammation at the third day post-infection. The immunosuppression resulted in body weight loss, leukopenia and reduced IL-2 production by spleen cell cultures. Higher fungal loads were recovered from the CNS of immunosuppressed mice. Inflammatory infiltration associated to a Th1 subset profile was higher in brain samples from C. krusei immunosuppressed mice compared with immunocompetent ones. Additionally, C. krusei was able to transform into pseudohypha inside microglia in vitro infected cells and also to induce elevated nitric oxide production. Altogether, these results indicate that C. glabrata, C. krusei and C. parapsilosis are able to disseminate to the CNS and promote local inflammation in both immunocompetent and immunosuppressed mice. C. krusei displayed a distinct behavior at the CNS triggering a local Th1 profile. The possible contribution of these non-albicans Candida species to other CNS pathologies as multiple sclerosis, Parkinson's and Alzheimer's diseases deserves further attention.en
dc.description.affiliationBotucatu Medical School São Paulo State University (UNESP)
dc.description.affiliationInstitute of Biosciences São Paulo State University (UNESP)
dc.description.affiliationUnespBotucatu Medical School São Paulo State University (UNESP)
dc.description.affiliationUnespInstitute of Biosciences São Paulo State University (UNESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipIdCNPq: 152790/2016-0
dc.description.sponsorshipIdCNPq: 307269/2017-5
dc.identifierhttp://dx.doi.org/10.3389/fmicb.2018.02968
dc.identifier.citationFrontiers in Microbiology, v. 10, n. JAN, 2019.
dc.identifier.doi10.3389/fmicb.2018.02968
dc.identifier.issn1664-302X
dc.identifier.lattes3320327570429539
dc.identifier.lattes8510423269540465
dc.identifier.orcid0000-0002-8003-4109
dc.identifier.orcid0000-0003-1380-7527
dc.identifier.scopus2-s2.0-85064415950
dc.identifier.urihttp://hdl.handle.net/11449/190264
dc.language.isoeng
dc.relation.ispartofFrontiers in Microbiology
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectCandida spp.
dc.subjectFungal infections
dc.subjectMicroglia
dc.subjectNeuroinflammation
dc.subjectPrednisolone
dc.titleDifferential behavior of non-albicans Candida species in the central nervous system of immunocompetent and immunosuppressed miceen
dc.typeArtigo
unesp.author.lattes4977572416129527[7]
unesp.author.lattes3320327570429539[6]
unesp.author.lattes8510423269540465[8]
unesp.author.orcid0000-0003-4557-3331[7]
unesp.author.orcid0000-0002-8003-4109[6]
unesp.author.orcid0000-0003-1380-7527[8]

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Artigos - Microbiologia e Imunologia - IBB
Artigos - Pediatria - FMB