Relationship between DNA damage and sperm head birefringence

Página do item simplificado
dc.contributor.authorPetersen, C. G. [UNESP]
dc.contributor.authorVagnini, L. D.
dc.contributor.authorMauri, A. L.
dc.contributor.authorMassaro, F. C.
dc.contributor.authorCavagna, M.
dc.contributor.authorBaruffi, R. L. R.
dc.contributor.authorOliveira, J. B. A. [UNESP]
dc.contributor.authorFranco, J. G. [UNESP]
dc.contributor.institutionCtr Human Reprod Prof Franco Jr
dc.contributor.institutionPaulista Ctr Diag Res & Training
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T13:35:28Z
dc.date.available2014-05-20T13:35:28Z
dc.date.issued2011-06-01
dc.description.abstractBirefringence or double refraction is the decomposition of a ray of light into two rays when it passes through an anisotropic material such as quartz. Sperm cells have been demonstrated to be optically anisotropic. The objective of this study was to evaluate the relationship between the pattern of human sperm head birefringence (SHBF) and DNA damage. A total of 26 patients with normal semen were included. DNA damage (fragmentation and denaturation) was evaluated in the sperm head in the context of birefringence, both total (SHBF-T) and partial (SHBF-P), by terminal deoxyribonucleotidyl transferase (TdT)-mediated dUDP nick-end labelling assay and acridine orange fluorescence, respectively. Positive DNA fragmentation in spermatozoa with SHBF-T (205/1053; 19.5%) was significantly higher (P < 0.0001) than in spermatozoa that presented SHBF-P (60/820; 7.3%). However, the percentage of denatured DNA in spermatozoa with SHBF-T (824/1256; 65.6%) was not significantly different from the ones with SHBF-P (666/1009; 66.0%). In conclusion, the data support a positive relationship between spermatozoa with total SHBF in their head and increased DNA fragmentation. (C) 2011, Reproductive Healthcare Ltd. Published by Elsevier Ltd. All rights reserved.en
dc.description.affiliationCtr Human Reprod Prof Franco Jr, Ribeirao Preto, Brazil
dc.description.affiliationPaulista Ctr Diag Res & Training, Ribeirao Preto, Brazil
dc.description.affiliationSão Paulo State Univ, Botucatu Med Sch, Dept Gynecol & Obstet, Botucatu, SP, Brazil
dc.description.affiliationUnespSão Paulo State Univ, Botucatu Med Sch, Dept Gynecol & Obstet, Botucatu, SP, Brazil
dc.description.sponsorshipResearch Support Group, UNESP
dc.format.extent583-589
dc.identifierhttp://dx.doi.org/10.1016/j.rbmo.2011.03.017
dc.identifier.citationReproductive Biomedicine Online. Oxford: Elsevier B.V., v. 22, n. 6, p. 583-589, 2011.
dc.identifier.doi10.1016/j.rbmo.2011.03.017
dc.identifier.issn1472-6483
dc.identifier.urihttp://hdl.handle.net/11449/12208
dc.identifier.wosWOS:000303042100015
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofReproductive BioMedicine Online
dc.relation.ispartofjcr2.967
dc.relation.ispartofsjr1,343
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectDNA denaturationen
dc.subjectDNA fragmentationen
dc.subjectMSOMEen
dc.subjectsperm birefringenceen
dc.subjectsperm DNA damageen
dc.subjectsperm selectionen
dc.titleRelationship between DNA damage and sperm head birefringenceen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina, Botucatupt

Arquivos

Licença do Pacote
Agora exibindo 1 - 2 de 2
Nenhuma Miniatura disponível
Nome:
license.txt
Tamanho:
1.71 KB
Formato:
Item-specific license agreed upon to submission
Descrição:
Nenhuma Miniatura disponível
Nome:
license.txt
Tamanho:
1.71 KB
Formato:
Item-specific license agreed upon to submission
Descrição:

Coleções

Artigos - Ginecologia e Obstetrícia - FMB