Effects of ethanol, acetaldehyde, and acetic acid on histamine secretion in guinea pig lung mast cells

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dc.contributor.authorRuiz, C. M.
dc.contributor.authorGomes, J. C.
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T13:48:59Z
dc.date.available2014-05-20T13:48:59Z
dc.date.issued2000-02-01
dc.description.abstractWe studied the direct effects of ethanol and its metabolites on the guinea pig lung mast cell, and the alterations caused in the histamine release induced by different stimuli. Guinea pig lungs cells dispersed by collagenase were used throughout. High concentrations of ethanol (100 mg/ml), acetaldehyde (0.3-3 mg/ml) and acetic acid (3 mg/ml) induced histamine release that was not inhibited by sodium cyanide (0.3 mM). Lower concentration of ethanol (10 mg/ml) and acetic acid (0.3 mg/ml), but not acetaldehyde, inhibited the histamine release induced by antigen and ionophore A23187. The histamine release induced by phorbol 12-miristate 13-acetate (1 mu M) was also inhibited by ethanol (10 mg/ml). Changes in the levels of calcium, glucose and phosphatidic acid did not influence the effect of ethanol. We conclude that high doses of ethanol, acetaldehyde, and acetic acid cause a cytotoxic histamine release by independent mechanisms. Low concentrations of acetic acid inhibit the histamine release by pH reduction. Ethanol acts by a generalized effect that is independent of calcium and glucose suggesting a nonspecific effect that, nevertheless, is not cytotoxic since it can be reversed by washing the cells. (C) 2000 Elsevier B.V. All rights reserved.en
dc.description.affiliationUNESP, Inst Biosci, Dept Pharmacol, BR-18618000 Botucatu, SP, Brazil
dc.description.affiliationUnespUNESP, Inst Biosci, Dept Pharmacol, BR-18618000 Botucatu, SP, Brazil
dc.format.extent133-138
dc.identifierhttp://dx.doi.org/10.1016/S0741-8329(99)00065-8
dc.identifier.citationAlcohol. Oxford: Pergamon-Elsevier B.V., v. 20, n. 2, p. 133-138, 2000.
dc.identifier.doi10.1016/S0741-8329(99)00065-8
dc.identifier.issn0741-8329
dc.identifier.urihttp://hdl.handle.net/11449/17434
dc.identifier.wosWOS:000085651000005
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofAlcohol
dc.relation.ispartofjcr2.425
dc.relation.ispartofsjr1,153
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjecthistamine releasept
dc.subjectmast cellspt
dc.subjectethanolpt
dc.subjectguinea pigpt
dc.titleEffects of ethanol, acetaldehyde, and acetic acid on histamine secretion in guinea pig lung mast cellsen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatupt

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