Type-2 diabetes mellitus and the frequency of the G22A polymorphism of the adenosine deaminase gene in a mixed population in Brazil

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dc.contributor.authorBonini Domingos, Ana Carolina [UNESP]
dc.contributor.authorIacida, Edi Carlos [UNESP]
dc.contributor.authorOliveira, Rafael Monteiro de [UNESP]
dc.contributor.authorBonini-Domingos, Claudia Regina [UNESP]
dc.contributor.authorBrandao de Mattos, Cinara de Cassia
dc.contributor.authorMattos, Luiz Carlos de
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionFaculdade de Medicina de São José do Rio Preto (FAMERP)
dc.date.accessioned2014-12-03T13:11:06Z
dc.date.available2014-12-03T13:11:06Z
dc.date.issued2014-07-01
dc.description.abstractMost individuals diagnosed with diabetes mellitus (about 90%) have type 2 disease (T2DM). T2DM is associated with a high genetic predisposition and is characterized by changes in the secretion and action of insulin. There have been reports of increased activity of the adenosine deaminase enzyme in individuals with coronary heart disease and DM.We evaluated 162 patients with T2DM and 160 individuals without the disease. Additionally, a subgroup of 81 individuals at higher risk of developing cardiovascular diseases was formed from the group of patients with diabetes on the basis of their serum levels of high-density lipoprotein cholesterol (HDLc), low-density lipoprotein cholesterol (LDLc) and triglycerides. PCR-RFLP was performed to analyze the TaqI polymorphism G428A of the ADA gene; this technique identifies the ADA*01 and ADA*02 alleles.Genotype frequencies were calculated for three patient groups, as follows. Patients with diabetes: ADA*01;*01 (142/159, 89.3%), ADA*01;*02 (16/159, 10.1%) and ADA*02;*02 (1/159, 0.6%); control group: ADA*01;*01 (146/160, 91.3%), ADA*01;*02 (12/160, 7.5%) and ADA*02;*02 (2/160, 1.3%); patients at risk of cardiovascular disease: ADA*01;*01 (71/78, 91.0%), ADA*01;*02 (7/78, 9.0%) and ADA*02;*02 (0/78, 0.0%).No statistically significant differences between the groups were observed in the genotype and allele frequencies. However, this fact does not rule out the need for further studies on the frequencies of this polymorphism in the Brazilian population, on any association with ADA enzyme activity, and on other risk factors that can affect the quality of life of Brazilian patients with T2DM.en
dc.description.affiliationUniv Estadual Paulista Julio de Mesquita Filho UN, Letras & Ciencias Exatas IBILCE, Inst Biociencias, Lab Hemoglobins & Genet Hematol Dis LHGDH,Dept Bi, BR-15054000 Sao Jose Do Rio Preto, SP, Brazil
dc.description.affiliationFac Med Sao Jose do Rio Preto FAMERP, Dept Mol Biol, Immunogenet Lab, BR-15054000 Sao Jose Do Rio Preto, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista Julio de Mesquita Filho UN, Letras & Ciencias Exatas IBILCE, Inst Biociencias, Lab Hemoglobins & Genet Hematol Dis LHGDH,Dept Bi, BR-15054000 Sao Jose Do Rio Preto, SP, Brazil
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipMedical School in Sao Jose do Rio Preto (BAP-FAMERP), Brazil
dc.format.extent439-443
dc.identifierhttp://dx.doi.org/10.1093/trstmh/tru076
dc.identifier.citationTransactions Of The Royal Society Of Tropical Medicine And Hygiene. Oxford: Oxford Univ Press, v. 108, n. 7, p. 439-443, 2014.
dc.identifier.doi10.1093/trstmh/tru076
dc.identifier.issn0035-9203
dc.identifier.lattes3279428066176719
dc.identifier.orcid0000-0002-4603-9467
dc.identifier.urihttp://hdl.handle.net/11449/112857
dc.identifier.wosWOS:000338134700010
dc.language.isoeng
dc.publisherOxford University Press
dc.relation.ispartofTransactions of the Royal Society of Tropical Medicine and Hygiene
dc.relation.ispartofjcr2.820
dc.relation.ispartofsjr1,174
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectAdenosine deaminase enzyme polymorphismen
dc.subjectRisk of cardiovascular diseaseen
dc.subjectType-2 diabetes mellitusen
dc.titleType-2 diabetes mellitus and the frequency of the G22A polymorphism of the adenosine deaminase gene in a mixed population in Brazilen
dc.typeArtigo
dcterms.licensehttp://www.oxfordjournals.org/access_purchase/self-archiving_policyb.html
dcterms.rightsHolderOxford Univ Press
unesp.author.lattes3279428066176719[4]
unesp.author.orcid0000-0002-4836-3113[5]
unesp.author.orcid0000-0002-8572-8177[6]
unesp.author.orcid0000-0002-4603-9467[4]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências Letras e Ciências Exatas, São José do Rio Pretopt

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