Circulating Extracellular Vesicles microRNAs Are Altered in Women Undergoing Preterm Birth

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dc.contributor.authorRamos, Bruna Ribeiro Andrade [UNESP]
dc.contributor.authorTronco, Júlia Abbade [UNESP]
dc.contributor.authorCarvalho, Márcio [UNESP]
dc.contributor.authorFelix, Tainara Francini [UNESP]
dc.contributor.authorReis, Patrícia Pintor [UNESP]
dc.contributor.authorSilveira, Juliano Coelho
dc.contributor.authorSilva, Márcia Guimarães [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionUniversity of Western São Paulo (UNOESTE)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2023-07-29T13:51:14Z
dc.date.available2023-07-29T13:51:14Z
dc.date.issued2023-03-01
dc.description.abstractPreterm labor (PTL) and preterm premature rupture of membranes (PPROM) lead to high perinatal morbidity/mortality rates worldwide. Small extracellular vesicles (sEV) act in cell communication and contain microRNAs that may contribute to the pathogenesis of these complications. We aimed to compare the expression, in sEV from peripheral blood, of miRNAs between term and preterm pregnancies. This cross-sectional study included women who underwent PTL, PPROM, and term pregnancies, examined at the Botucatu Medical School Hospital, SP, Brazil. sEV were isolated from plasma. Western blot used to detect exosomal protein CD63 and nanoparticle tracking analysis were performed. The expression of 800 miRNAs was assessed by the nCounter Humanv3 miRNA Assay (NanoString). The miRNA expression and relative risk were determined. Samples from 31 women—15 preterm and 16 term—were included. miR-612 expression was increased in the preterm groups. miR-612 has been shown to increase apoptosis in tumor cells and to regulate the nuclear factor κB inflammatory pathway, processes involved in PTL/PPROM pathogenesis. miR-1253, miR-1283, miR378e, and miR-579-3p, all associated with cellular senescence, were downregulated in PPROM compared with term pregnancies. We conclude that miRNAs from circulating sEV are differentially expressed between term and preterm pregnancies and modulate genes in pathways that are relevant to PTL/PPROM pathogenesis.en
dc.description.affiliationDepartment of Pathology Botucatu Medical School São Paulo State University (UNESP), SP
dc.description.affiliationFaculty of Medicine—Jaú Campus University of Western São Paulo (UNOESTE), SP
dc.description.affiliationFaculty of Veterinary Medicine and Animal Science São Paulo State University (UNESP), SP
dc.description.affiliationExperimental Research Unity (UNIPEX) Botucatu Medical School São Paulo State University (UNESP), SP
dc.description.affiliationDepartment of Surgery and Orthopedics Botucatu Medical School São Paulo State University (UNESP), SP
dc.description.affiliationDepartment of Veterinary Medicine Faculty of Animal Science and Food Engineering São Paulo University (USP), SP
dc.description.affiliationUnespDepartment of Pathology Botucatu Medical School São Paulo State University (UNESP), SP
dc.description.affiliationUnespFaculty of Veterinary Medicine and Animal Science São Paulo State University (UNESP), SP
dc.description.affiliationUnespExperimental Research Unity (UNIPEX) Botucatu Medical School São Paulo State University (UNESP), SP
dc.description.affiliationUnespDepartment of Surgery and Orthopedics Botucatu Medical School São Paulo State University (UNESP), SP
dc.identifierhttp://dx.doi.org/10.3390/ijms24065527
dc.identifier.citationInternational Journal of Molecular Sciences, v. 24, n. 6, 2023.
dc.identifier.doi10.3390/ijms24065527
dc.identifier.issn1422-0067
dc.identifier.issn1661-6596
dc.identifier.scopus2-s2.0-85152691951
dc.identifier.urihttp://hdl.handle.net/11449/248703
dc.language.isoeng
dc.relation.ispartofInternational Journal of Molecular Sciences
dc.sourceScopus
dc.subjectinflammation
dc.subjectmicroRNAs
dc.subjectpreterm birth
dc.subjectpreterm labor
dc.subjectpreterm premature rupture of membranes
dc.subjectsmall extracellular vesicles
dc.titleCirculating Extracellular Vesicles microRNAs Are Altered in Women Undergoing Preterm Birthen
dc.typeArtigo
unesp.author.orcid0000-0003-4783-0573[2]
unesp.author.orcid0000-0003-3775-3797[5]
unesp.author.orcid0000-0002-4796-6393[6]

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Artigos - Patologia - FMB
Artigos - Cirurgia e Ortopedia - FMB