Hyperglycemic condition during puberty increases collagen fibers deposition in the prostatic stroma and reduces MMP-2 activity

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dc.contributor.authorSantos, Sérgio Alexandre Alcantara dos [UNESP]
dc.contributor.authorPorto Amorim, Elaine Manoela
dc.contributor.authorRibeiro, Larissa Mayume [UNESP]
dc.contributor.authorRinaldi, Jaqueline Carvalho
dc.contributor.authorDelella, Flávia Karina
dc.contributor.authorJustulin, Luis Antonio [UNESP]
dc.contributor.authorFelisbino, Sérgio Luis [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionState University of West Paraná (UNIOESTE)
dc.contributor.institutionUniversidade Estadual de Maringá (UEM)
dc.date.accessioned2018-12-11T17:34:26Z
dc.date.available2018-12-11T17:34:26Z
dc.date.issued2017-12-02
dc.description.abstractPuberty is an important period for the growth and maturation of the male reproductive system, and is also a critical window for endocrine or environmental interference. The physiological levels of circulating insulin and hyperglycemic control are important factors for a normal prostate growth. Hyperglycemia during puberty is reported to retard the growth of the prostate gland, with remarkable effects on the epithelial compartment. Here, we investigated the impact of hyperglycemia along with a simultaneous or late insulin replacement on the ventral prostate growth in rats during puberty, paying special attention to the deposition of collagen fibers and activities of gelatinase, matrix metalloproteinase-2 (MMP-2), and −9 (MMP-9). Hyperglycemia was induced by streptozotocin (STZ) administration in 40-day-old male Wistar rats. A subset of hyperglycemic rats underwent an early insulin replacement (three days after the STZ administration), and another subset underwent a late insulin replacement (twenty days after the STZ administration). Animals were euthanized at 60 and/or 80 days of age. The ventral prostatic lobe was processed for picrosirius red staining, type I and III collagen immunohistochemistry, and gelatin zymography. Hyperglycemic animals showed an increased area of collagen fibers in the prostate, which was composed both types of collagens. MMP-2 activity was significantly reduced in the hyperglycemic animals, while MMP-9 activity was very low and showed no alteration. The simultaneous and late insulin administration restored collagen content and MMP-2 activity. In conclusion, puberty is a critical window for prostate maturation and type-1 diabetes-induced hyperglycemia affects the ratio of the prostatic parenchymal and stromal growth, leading to fibrotic tissues by also MMP-2 down regulation.en
dc.description.affiliationDepartment of Morphology Institute of Biosciences Sao Paulo State University (UNESP)
dc.description.affiliationCenter of Biological and Health Sciences (CCBS) State University of West Paraná (UNIOESTE)
dc.description.affiliationDepartment of Morphological Sciences Biological Sciences Center State University of Maringa (UEM)
dc.description.affiliationUnespDepartment of Morphology Institute of Biosciences Sao Paulo State University (UNESP)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipIdFAPESP: 06/60116-9
dc.description.sponsorshipIdFAPESP: 2006/60115-2
dc.description.sponsorshipIdCNPq: 305391/2014-3
dc.format.extent1581-1586
dc.identifierhttp://dx.doi.org/10.1016/j.bbrc.2017.10.022
dc.identifier.citationBiochemical and Biophysical Research Communications, v. 493, n. 4, p. 1581-1586, 2017.
dc.identifier.doi10.1016/j.bbrc.2017.10.022
dc.identifier.file2-s2.0-85030834307.pdf
dc.identifier.issn1090-2104
dc.identifier.issn0006-291X
dc.identifier.scopus2-s2.0-85030834307
dc.identifier.urihttp://hdl.handle.net/11449/179264
dc.language.isoeng
dc.relation.ispartofBiochemical and Biophysical Research Communications
dc.relation.ispartofsjr1,087
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectCollagen
dc.subjectDiabetes
dc.subjectHyperglycemia
dc.subjectMetalloproteinase
dc.subjectProstate
dc.subjectPuberty
dc.titleHyperglycemic condition during puberty increases collagen fibers deposition in the prostatic stroma and reduces MMP-2 activityen
dc.typeArtigo
unesp.author.orcid0000-0002-1375-1634[1]
unesp.author.orcid0000-0002-1736-5984[4]

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