First description of the karyotype and localization of major and minor ribosomal genes in Rhoadsia altipinna Fowler, 1911 (Characiformes, Characidae) from Ecuador

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dc.contributor.authorSánchez-Romero, Omar
dc.contributor.authorAbad, César Quezada
dc.contributor.authorCordero, Patricio Quizhpe
dc.contributor.authorde Sene, Viviani França [UNESP]
dc.contributor.authorNirchio, Mauro
dc.contributor.authorOliveira, Claudio [UNESP]
dc.contributor.institutionEl Oro
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionEstado Nueva Esparta
dc.contributor.institutionUniversidad Nacional Mayor de San Marcos UNMSM
dc.date.accessioned2018-12-11T17:04:37Z
dc.date.available2018-12-11T17:04:37Z
dc.date.issued2015-01-01
dc.description.abstractKaryotypic features of Rhoadsia altipinna Fowler, 1911 from Ecuador were investigated by examining metaphase chromosomes through Giemsa staining, C-banding, Ag-NOR, and two-color-fluorescence in situ hybridization (FISH) for mapping of 18S and 5S ribosomal genes. The species exhibit a karyotype with 2n = 50, composed of 10 metacentric, 26 submetacentric and 14 subtelocentric elements, with a fundamental number FN=86 and is characterized by the presence of a larger metacentric pair (number 1), which is about 2/3 longer than the average length of the rest of the metacentric series. Sex chromosomes were not observed. Heterochromatin is identifiable on 44 chromosomes, distributed in paracentromeric position near the centromere. The first metacentric pair presents two well-defined heterochromatic blocks in paracentromeric position, near the centromere. Impregnation with silver nitrate showed a single pair of Ag-positive NORs localized at terminal regions of the short arms of the subtelocentric chromosome pair number 12. FISH assay confirmed these localization of NORs and revealed that minor rDNA clusters occur interstitially on the larger metacentric pair number 1. Comparison of results here reported with those available on other Characidae permit to hypothesize that the presence of a very large metacentric pair might represent a unique and derived condition that characterize one of four major lineages molecularly identified in this family.en
dc.description.affiliationUniversidad Técnica de Machala El Oro
dc.description.affiliationLaboratório de Biologia e Genética de Peixes Instituto de Biociências de Botucatu Universidade Estadual Paulista (UNESP) Departamento de Morfologia Distrito de Rubião Junior
dc.description.affiliationEscuela de Ciencias Aplicadas del Mar Universidad de Oriente Estado Nueva Esparta
dc.description.affiliationUniversidad Nacional Mayor de San Marcos UNMSM
dc.description.affiliationUnespLaboratório de Biologia e Genética de Peixes Instituto de Biociências de Botucatu Universidade Estadual Paulista (UNESP) Departamento de Morfologia Distrito de Rubião Junior
dc.format.extent271-280
dc.identifierhttp://dx.doi.org/10.3897/CompCytogen.v9i2.4504
dc.identifier.citationComparative Cytogenetics, v. 9, n. 2, p. 271-280, 2015.
dc.identifier.doi10.3897/CompCytogen.v9i2.4504
dc.identifier.issn1993-078X
dc.identifier.issn1993-0771
dc.identifier.scopus2-s2.0-84979896205
dc.identifier.urihttp://hdl.handle.net/11449/173315
dc.language.isoeng
dc.relation.ispartofComparative Cytogenetics
dc.relation.ispartofsjr0,431
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subject18S and 5S ribosomal genes
dc.subjectC-bands
dc.subjectfishes
dc.subjectkaryotype
dc.subjectNORs
dc.titleFirst description of the karyotype and localization of major and minor ribosomal genes in Rhoadsia altipinna Fowler, 1911 (Characiformes, Characidae) from Ecuadoren
dc.typeArtigo

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